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EC number: 443-010-4 | CAS number: 53641-10-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-12-03 to 2000-05-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 443-010-4
- EC Name:
- -
- Cas Number:
- 53641-10-4
- Molecular formula:
- C14H15ClN2O4
- IUPAC Name:
- N-[3-chloro-4-(3-oxobutanamido)phenyl]-3-oxobutanamide
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- Test item concentrations have been analytically verified at test initiation and test end
- Details on sampling:
- Separate replicates for the test item analysis after 0 and 72 h were prepared (test beginning provided without alga and test end with same density of alga as the test replicates) and incubated under test conditions.
Test solutions
- Vehicle:
- no
- Remarks:
- No vehicle was used to dissolve the test item. The test item was completely dissolved in all test replicates throughout the test period.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Scenedesmus subspicatus CHODAT SAG 86.81
Reason for selection: According to the guideline Scenedesmus subspicatus CHODAT SAG 86.81 is suitable for this kind of study.
Origin: Collection of algae cultures (SAG). Pflanzenphysiologisches Institut der Universität, Nikolausberger Weg 18, 0-37073 Göttingen
Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted 35-70 pE/m2 s for 24 h per day.
Culture medium: Nutrient medium Z according to LÜTTGE et al. (1994).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- Mean: 23.8 °C
- pH:
- 7.47 - 8.11
- Nominal and measured concentrations:
- Nominal: 15.625, 31.25, 62.5, 125. 250, 500, 1000 mg/L
a.i. nominal: 15.40625, 30.8125, 61.625, 123.25, 246.5, 493, 986 mg/L
Measured: 80-93% of nominal - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 219 mg/L
- 95% CI:
- 195 - 246
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 450 mg/L
- 95% CI:
- 396 - 512
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Microscopic evaluation of the cells at the start of the incubation period revealed no morphological abnormalities. At the end of the test cells at concentration levels 500 and 1000 mg/L were lighter green and at concentration level 1000 mg/L cells were smaller than in the control. Water quality parameters of pH-value, measured at 0 and 72 h, and room temperature, measured continuously, met the guideline requirements.
- Reported statistics and error estimates:
- Calculations were carried out using software
- SigmaPlot rel. 5.0 (1986-1995), JANDEL CORPORATION
- SigmaStat rel. 2.03 (1992-1997), SPSS INC.
- Excel rel. 5.0a (1985 - 1993), MICROSOFT CORPORATION
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study CL-DAEP was found to inhibit the growth of the freshwater green alga
Scenedesrnus subspicatus at concentrations > 15.625 mg/L (biomass and growth rate).
The EC50-values for inhibition of biomass growth (E,C„) and specific growth rate (E,C50) after
72 h were 219 (195 - 246) and 450 (396 - 512) mg/L, respectively. - Executive summary:
The acute toxicity of CL-DAEP batch no. 90003 CCL to the unicellular freshwater green alga Scenedesmus subspicatus was determined according to the principles of OECD-Guideline 201 at DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE in 31157 Sarstedt, Germany from February 29 to March 3, 2000.
The study was conducted under static conditions over a duration of 72 hours with an initial cell density of nominally 104 cells/mL. 7 concentration levels were tested in a geometrical series with a dilution factor of 2:
15.625 - 31.25 - 62.5 - 125 - 250 - 500- 1000 mg/L.3 replicates were tested for each concentration and 6 replicates for control.
No vehicle was used to dissolve the test item. The test item was completely dissolved in all test replicates throughout the test period.
Microscopic evaluation of the cells at the start of the incubation period revealed no morphological abnormalities. At the end of the test cells at concentration levels 500 and 1000 mg/L were lighter green and at concentration level 1000 mg/L cells were smaller than in the control. Water quality parameters of pH-value, measured at 0 and 72 h, and room temperature, measured continuously, were determined to be within the acceptable limits.
The concentrations of the active ingredient 2-Chlor-bis-(N-acetoacetyI)-p-phenylendiamin were determined at all concentration levels at test start and test end. The overall mean values of recovery rates of the active ingredient at the beginning and the end of the test were > 80 % (except one dosage level with 79 %). Therefore all effect concentrations are based on nominal concentrations of the test item.The effects of CL-DAEP, based on the nominal concentrations are:
Biomass:
72h EC50 = 219 mg/L (CI: 195-246 mg/L)
72h NOEC = 15.625 mg/L
Growth rate:
72h EC50 = 450 mg/L (CI = 396-512 mg/L)
72h NOEC = 15.625 mg/L
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