Disodium 1,5-dihydroxypentane-1,5-disulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 14 NOVEMBER to 20 NOVEMBER 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

According to OECD 202 resp. EU C.2

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 1,5-dihydroxypentane-1,5-disulphonate

Cas Number:

7420-89-5

Molecular formula:

C5H10Na2O8S2

IUPAC Name:

Disodium 1,5-dihydroxypentane-1,5-disulphonate

impurity 1

Reference substance name:

Sodium sulfate

Cas Number:

7757-82-6

IUPAC Name:

Sodium sulfate

impurity 2

Reference substance name:

Sodium hydrogen sulfite

Cas Number:

7631-90-5

IUPAC Name:

Sodium hydrogen sulfite

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Young daphnia, aged less than 24 hours at the start of the test

Study design

Test type:

static

Water media type:

other: CaCl2*2H2O: 293.80 mg/l, MgSO4*7H2O: 123.30 mg/l, NaHCO3:64.80 mg/l, KCl: 5.80 mg/l

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

Immobilisation was recorded at 24 hours and 48 hours and compared with control values

Test conditions

Hardness:

Resulting hardness in mmol/L: 2.502
Resulting hardness in mg CaCO3/L: 250

pH:

7.8

Reference substance (positive control):

yes

Remarks:

Potassium dichromate was used as positive control in a current reference study. The 24h-EC50i value was determined as 1.9 mg/L. The value was within the requested range between 0.6 - 2.1 mg/L of potassium dichromate as specified in the OECD guideline.

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In the blank control and in the treatment, none of the Daphnia was immobilised.
Results Test Item:

48h-NOEC = ≥ 100 mg/L (nominal)
48h-LOEC = > 100 mg/L (nominal)
24h-EC50 = > 100 mg/L (nominal)
48h-EC50 = > 100 mg/L (nominal)

Results with reference substance (positive control):

Potassium dichromate K2Cr2O7 was used as positive control in a current reference study. The 24h-EC50i value was determined as 1.9 mg/L. The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium dichromate K2Cr2O7 as specified in the OECD guideline.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results were determined for the test item Glutardialdehyde bis-Sodiumbisulfite (species: Daphnia magna).

48h-NOEC = ≥ 100 mg/L (nominal)
48h-LOEC = > 100 mg/L (nominal)
24h-EC50 = > 100 mg/L (nominal)
48h-EC50 = > 100 mg/L (nominal)

Executive summary:

One valid experiment was performed.

The study was performed as a limit test at the concentration100 mg/L (nominal). For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

None of the animals was immobilised in the treatment and the blank control. Therefore no statistical evaluation was performed.

Potassium dichromate K₂Cr₂O₇ was used as positive control in a current reference study to assure that the test conditions are reliable.

 

At the start and at the end of the test, the content of the test item in the test solutions was determined using measurement of dissolved organic carbon (DOC). The measured concentration based on DOC measurement was 90 % of the nominal concentration at the beginning of the test and 110 % of the nominal concentration at the end of the test. Therefore, the determination of the results was based on the nominal concentrations.