Propanenitrile, 2-[bis(cyanomethyl)amino]-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Name of test substance: MGDN
Batch No: Gre 3038/F535
Degree of purity: 97.9%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Bio International B.V. NJ Horn, Netherlands
- Age at study initiation: About 3 months
- Length at study initiation: 3.32 (2.5-4.2) cm
- Weight at study initiation: 0.203 (0.1 -0.39) g

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Remarks on exposure duration:

h

Test conditions

Hardness:

Total hardness: about 2.5 mmol/L (= about 250 mg/L CaCO3)

Test temperature:

22 °C

pH:

About 8.0 - 8.6

Dissolved oxygen:

Slight aeration

Nominal and measured concentrations:

Test concentrations (mg/L)
nominal / measured after 1 h / measured after 96 h:
10 / 10.5 (105%) / 9.7 (97%)
21.5 / 21.9 (101.9 %) / 21.2 (98.6%)
46.4 / 46.6 (100.4%) / 46.2 (99.6%)
100 / 99.9 (99.9%) / 98.4 (98.4%)
215 / 212 (98.6)

Details on test conditions:

TEST SYSTEM
- Test vessel: All-glass aquaria (30 x 22 x 24 cm)
- Volume of water: 10 liters (water depth about 17 cm)
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- Biomass loading rate: 0.2 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, aerated
- Ca content: about 90 mg/L
- Mg content: about 10 mg/L

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

EFFECT PARAMETERS MEASURED:
- Determination of mortality and symptoms
- Observation intervals: 1, 4, 24, 48, 72, 96 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

21.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

46.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 46.2 - < 98.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

98.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Test results 96h measured:
NOEC = 21.5 mg/L
LC0 = 46.4 mg/L
LC 100 = 100 mg/L