Decyl dihydrogen phosphate, potassium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10-14 September 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Good quality study under GLP, however no chemical analyses and no certificate of analysis or detailed description of the test substance.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

no chemical analyses

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

no chemical analyses

Details on test solutions:

Direct dispersion in water. 4.0 g of test material was dispersed into water and the volume adjusted to 1 litre. 500 mL of this stock solution was dispersed in a final volume of 20 litres of diluent to give the test concentration of 100 mg/L.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Rainbow trout (Oncorhynchus mykiss)
Source: Parkwood trout farm, Wigmore, kent, United Kingdom.
The stock of fish was held since 16 July 1991 and was acclimatised to test conditions from 2 -10 September 1991.
The stock fish were maintained in a glass fibre tank with a single-pass water renewal system. The light was controlled to give a 16h light and 8h
darkness cycle. Fish were fed dailey with commercial trout pellets, but feeding was discontinued 24h prior to the test.
Mean standard length = 4.9 cm (SD = 0.3 cm), mean weight = 1.55 g (SD = 0.26 g), no data on age of fish.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96

Remarks on exposure duration:

h

Post exposure observation period:

no

Hardness:

Approximately 50 mg/L as CaCO3

Test temperature:

14.0 ºC

pH:

7.3-7.4

Dissolved oxygen:

10.0 - 10.1 mg O2/L

Nominal and measured concentrations:

Nominal concentration: 100 mg/L (40 mg/L active ingredient)

Details on test conditions:

The test material was suspected to absorb to glassware and so saturation of the absorption sites was achieved by soaking the test vessels overnight prior to the start of the test with the test solutions. At 0 hours the test vessels were emptied, rinsed with the solution to be tested and then refilled
with the fresh test solution.
There was a dailey renewal of test media to ensure stability of the test concentrations.

The lightning was controlled at 16h light- 8 h darkness cycle.

Test vessels: Glass aquaria holding 20L of test media. 1 test concentration in duplicate, plus 1 control (20 animals per test concentration).
Fish were placed at random in prepared test media, loading 0.78 g bodyweight/L.
Aeration via narrow bore glass tubes.

Criteria of death: absence of (i) respiratory movement and (ii) response to a physical stimulation.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 40 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

No mortality or behavioural effects were observed at the test concentration of nominally 100 mg/L at any time during the course of the study.
The NOEC can therefore be concluded at 100 mg/L, and the LC50 (96h) >= 100 mg/L.

Results with reference substance (positive control):

no

Sublethal observations / clinical signs:

no mortality throughout the study

Validity criteria fulfilled:

yes

Conclusions:

Good quality study, however no chemical analyses therefore concentrations nominal.

Executive summary:

TEST SPECIES: Rainbow trout (Oncorhynchus mykiss)

TEST TYPE: Acute toxicity 96 h LC50

TEST CONDITIONS: Semi-static test conditions accordi ng to OECD Guideline No. 203 referenced as Method C. 1 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/584/EEC)

RESULTS:

LC50 (96h) >100mg/L > 40mg/L ai

ai = active ingredient "No Observed Effect Concentration" (NOEC) >100 mg/l (>40 mg ai / l ) It was considered unnecessary and

unrealistic to test at concentrations in excess o f 100 mg/l

Description of key information

In a Semi-static test according to OECD Guideline No. 203 referenced as Method C. 1 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/584/EEC) the

LC50 (96h) >100mg/L > 40mg/L ai was determined

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

40 mg/L