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EC number: 268-859-6 | CAS number: 68152-93-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Range finding test: 10-14 June 2013 First definitive test: 12-16 August 2013 Second definitive test: 2-6 September 2013 Third definitive test: 16-20 September 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Version / remarks:
- The method followed for this study was cefas SOP 2113: "A 72h growth inhibition test for the marine alga Skeletonema costatum (GLP)", based on the guideline ISO 10253(2006)
- Deviations:
- yes
- Remarks:
- During the study, five deviations from the study plan occurred. The deviations were determined not to have affected the validity of the study.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Initially a solubility study was attempted using the whole product EnvaMul 600. However, as the substance is extremely difficult to transfer it was not possible to dissolve in seawater and was treated as insoluble. As EnvaMul 600 contains a mixture of components test solutions were prepared as water accomodated fractions (WAFs). A WAF is an aqueous medium containing only that fraction of a substance which remain in the aqueous phase after the preparation procedure is terminated. WAFs are prepared in glass aspirators with a tap at the bottom and a bung in the top to prevent loss of volatiles.
- Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- Test organism : Skeletonema costatum CCAP strain 1077/5. The original culture was obtained from Brixham Environmental laboratory.
- Test type:
- static
- Water media type:
- other: natural seawater, filteres to 0.2 µm collected from Lowestoft bay
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20.0 - 21.0 °C
- pH:
- 7.5 - 8.2
- Salinity:
- 31.7 - 32.4 %
- Nominal and measured concentrations:
- 10, 22, 46, 100 and 220 mg/L
- Details on test conditions:
- Light: constant 6710 - 9550 lux
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 51.4 mg/L
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 10 mg/L
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- EC50 after 72 h for reference test substance (3,5-dichlorophenol) was 0.84 mg/L (confidence limits:0.64 - 1.11). The treshold criteria are between 0.7 - 2.5 mg/L. The validity criteria are met.
- Reported statistics and error estimates:
- The data were analysed using CETIS statistical software and following Cefas SOP 2100: "The use of the statistical software CETIS"
The EC50 was calculated by linear interpolation and the LOEC and NOEC were calculated using the Wilcoxon/Bonferroni adjusted test. - Validity criteria fulfilled:
- yes
- Conclusions:
- The median lethal concentration (EC50) of test item Envamul 600 was 51.4 mg/L, with 95% confidence limits between 46.5 to 55.2 mg/L over 72 hours.
In addition, the No Observed Effect Concentration (NOEC was <10 mg/L and the Lowest Observed Effect Concentration (LOEC) was 10 mg/L - Executive summary:
A 72 hour acute toxicity assay was conducted with the test item ENVAMUL 600 using the marine algae Skeletonema costatum. The method followed for this study was cefas SOP 2113: "A 72h growth inhibition test for the marine alga Skeletonema costatum (GLP)", based on the guideline ISO 10253(2006).
A solubility study was attempted using the whole product EnvaMul 600, however as the substance is extremely difficult to transfer it was not possible to dissolve in seawater and was treated as insoluble. A Water Accommodated Fraction (WAF) was prepared and used to create the test concentrations (dilution water: natural seawater, filtered to 0.2 µm).
A rangefinder test and a definitive test were conducted.
The definitive study was conducted with 3 replicates per concentration with 6 reps for the control. According to the rangefinding test results, the test concentrations were chosen as 0 (control), 10, 22, 46, 100 and 220 mg/L Envamul 600. The test was conducted in 250 ml glass conical flasks as a static exposure for 72 hours.
RESULTS:
EC50: 51.4 mg/L
NOEC: <10 mg/L
LOEC: 10 mg/L
Reference
Description of key information
A 72 hour acute toxicity assay was conducted using the marine algae Skeletonema costatum. The method followed for this study was cefas SOP 2113: "A 72h growth inhibition test for the marine alga Skeletonema costatum (GLP)", based on the guideline ISO 10253(2006).
According to the range-finding test results, the test concentrations were chosen as 0 (control), 10, 22, 46, 100 and 220 mg/L Envamul 600. The test was conducted in 250 ml glass conical flasks as a static exposure for 72 hours.
RESULTS:
EC50: 51.4 mg/L
NOEC: <10 mg/L
LOEC: 10 mg/L
Key value for chemical safety assessment
- EC50 for marine water algae:
- 51.4 mg/L
- EC10 or NOEC for marine water algae:
- 10 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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