N,N,N',N',N'',N''-hexamethyl-4,4',4''-methylidynetrianiline

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38 412 "Testverfahren mit Wasseroganismen (GruppeL). Allgemeine Hinweise zur Planung, Durchfuehrung und Auswertung Biologischer Test-Verfahren (L1)" und "Bestimmung der Wirkung von Wasserinhaltsstoffen auf Fische - Fischtest (L15)", June 1982

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N,N,N',N',N'',N''-hexamethyl-4,4',4''-methylidynetrianiline

EC Number:

210-043-9

EC Name:

N,N,N',N',N'',N''-hexamethyl-4,4',4''-methylidynetrianiline

Cas Number:

603-48-5

Molecular formula:

C25H31N3

IUPAC Name:

4-{bis[4-(dimethylamino)phenyl]methyl}-N,N-dimethylaniline

Test material form:

solid

Details on test material:

- Purity: technical

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

water

Details on test solutions:

The test substance was added to the test water without any pre-treatment.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus idus L., Golden variety
- Source: Fischzucht Paul Eggers, D-2354 Hohenwestedt, FRG
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): 6.2 cm (5.7-6.9 cm)
- Weight at study initiation (mean and range, SD): 2.7 g (2.2-3.4 g)
- Method of breeding: not reported
- Maintenance of the brood fish: The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Medical treatment: Twice with 0.05 mg/L malachite green chloride and once with 10 mg/L tetracycline hydrochloride

ACCLIMATION
- Acclimation period: ~ 6 weeks
- Acclimation conditions (same as test or not): Same
- Type and amount of food during acclimation: Growing feed F/B 50 (Ssniff Spezialdiaeten GMBH, D-4770 Soest, FRG)
- Feeding frequency during acclimation: ad libitum
- Health during acclimation (any mortality observed): No mortality was observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No

Test conditions

Hardness:

~ 2.5 mmol/L

Test temperature:

In the test substance vessel, 21°C at start of test, 21°C at 24 hours, 20°C at 48 hours, 21°C at 72 and 96 hours and In the control vessel, 21°C at start of test, 21°C at 24 hours, 20°C at 48 hours, 21°C at 72 and 96 hours

pH:

In the test substance vessel, 8.0 at start of test and at 24, 48, 72, and 96 hours and In the control vessel, 8.0 at start of test, 8.0 at 24 hours, 8.0 at 48 hours, and 7.9 at 72 and 96 hours

Dissolved oxygen:

In the test substance vessel, 7.4 mg/L at start of test, 8.1 mg/L at 24 hours, 8.3 mg/L at 48 hours, 8.2 mg/L at 72 hours, and 8.0 mg/L at 96 hours and In the control vessel, 7.8 mg/L at start of test, 6.7 mg/L at 24 hours, 7.4 mg/L at 48 hours, 7.2 mg/L at 72 hours, and 7.6 mg/L at 96 hours

Conductivity:

Max. 10 micro MHO

Nominal and measured concentrations:

10000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquarium (30 cm x 22 cm x 24 cm)
- Material, size, headspace, fill volume: 10 litres
- Aeration: Aerated with oil-free air
- Type of flow-through (e.g. peristaltic or proportional diluter): Static
- Renewal rate of test solution (frequency/flow rate): No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 2.7

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412, part 11, Draft Sept. 1981 preparation from fully demineralized tap water
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No
- Intervals of water quality measurement: not reported

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The test solution was cloudy and coloured. Undissolved test substance was visible on the water surface. No mortality or adverse symptoms were observed at any time point.

Results with reference substance (positive control):

Positive control of animals was conducted with chloroacetamide. LC50 after 48 hours: about 31 mg/L (determined Nov. 30, 1987)

Reported statistics and error estimates:

Calculation of the median lethal concentration (LC50) and, if possible, the LC5 and the LC95 were determined using probit analysis.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Fish mortality after 1, 4, 24, 48, 72, and 96 hours of exposure

 

Concentration	Number of fish	Exposure time	Mortality	% Mortality
0	10	1 4 24 48 72 96	0 0 0 0 0 0	0 0 0 0 0 0
10000 mg/L	10	1 4 24 48 72 96	0 0 0 0 0 0	0 0 0 0 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 was greater than 10000 mg/L, the highest concentration tested.

Executive summary:

Groups of 10 golden orfe fish, Leuciscus idus L., were exposed for 96 hours to 0 or 10000 mg/L of the test substance dissolved in water. There was no mortality and no signs of toxicity.