Hexane-1,2,6-triol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09.03.1989 to 08.05.1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EEC Guideline 84/449/EEC

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hexane-1,2,6-triol

EC Number:

203-424-6

EC Name:

Hexane-1,2,6-triol

Cas Number:

106-69-4

Molecular formula:

C6H14O3

IUPAC Name:

hexane-1,2,6-triol

Test material form:

other: Liquide

Test animals / tissue source

Species:

rabbit

Strain:

other: White russian (Albino)

Details on test animals or tissues and environmental conditions:

TEST ANIMALS:
- Strain: Rabbit / White Russian
- Breeder: ASTA Pharma AG, D-4800 Bielefeld 14
- Number and sex: 3 animals (1 males and 2 females)
- Body weigth: male 2.61 kg, females 2.65 or 2.96 kg
- Age: 9 or 10 month
- Acclimation period: 14 days

ENVIRONMENTAL CONDITIONS:
- Diet: Standard diet, ssniff K, "Special Diet for Rabbits" supplied by ssniff Spezialfutter GmbH, D-4770 Soestad libitum
- Water: tab water ad libitum
- Housing individually in stainless steel cages
- Temperature: 18.5-24°C
- Rel. Humidity: 40-65%
- Light/dark period: 12/12h

Test system

Vehicle:

water

Duration of treatment / exposure:

The treated eye was examined using a Cliptrix pencil light (supplied by Varta, Hannover, No. 645). Signs of irritation on the cornea, iris and conjunctiva were assessed quantitatively and qualitatively using the DRAIZE scale. In addition, attention was paid to the occurrence of further reactions on the eye (e.g. corrosive effect) and to clinical symptoms (systemic toxic effects).

Observation period (in vivo):

Observation Times: 1, 24, 48 and 72 hours p. appl.
Duration of the Observation Period: 3 days p. appl.

Number of animals or in vitro replicates:

1 male and 2 females

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

2

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible

Results 4

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

3

Results 5

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

3

Results 6

Irritation parameter:

chemosis score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritant / corrosive response data:

The single instillation of 0.1 ml 1,2,6-Hexantriol into one conjunctival sac each of three rabbits caused only slight changes in the conjunctiva. The cornea and the iris were without any findings.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the conditions of this test, the test item is non-irritant to the rabbit eye. Thus, 1,2,6-Hexantriol is considered to be non-irritant in this test system, and according to Regulation (EC) 1272/2008 the data are conclusive but not sufficient for classification.