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EC number: 229-904-5 | CAS number: 6829-22-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Macrolex Rot E2G (CAS no 6829-22-7) was investigated for point mutagenic effects in the Salmonella/microsome test (plate incorporation test). The test item, dissolved in DMSO, was administered in doses of up to and including 5000 µg per plate without and with S9 mix on the five Salmonella typhimurium LT2 mutant strains TA1535, TA100, TA1537, TA98 and TA102, according to the OECD guideline 471.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one
- EC Number:
- 229-904-5
- EC Name:
- 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one
- Cas Number:
- 6829-22-7
- Molecular formula:
- C22H12N2O
- IUPAC Name:
- 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- Test Item: Macrolex Rot E2G
CAS No.: 6829-22-7
Molecular Formula: C22H12N20
Chemical Name: 14H-Benz[4,5]isoquino[2, 1-a]perimidin-14-one
CAS Name: 14H-Benz[4,5]isoquino[2, 1-a]perimidin-14-one
Molecular Mass (g/mol): 320.3
Purity: 98.9%
Appearance: red powder
Storage: room temperature
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Details on mammalian cell type (if applicable):
- The original strains were obtained from Prof. Bruce Ames (Berkeley, CA, USA). TA1535 and TA100 bear the base-pair substitution, his G 46, and TA100 additionally contains the plasmid pKM 101. This R factor also contained in TA98 and TA102, codes for an ampicillin resistance and should raise the sensitivity of the strains. TA102 carries the ochre mutation his G 428 on the multicopy plasmid pAQl, which codes in addition for tetracycline resistance. TA1537 and TA98 bear frameshift markers. TA1537 exhibits the +1 mutant, his C 3076, while TA98 bears the +2 type, his D 3052.
Furthermore, the strains have other properties, which should increase their sensitivity. They are all deep rough, i.e. partly deficient in lipopolysaccharide side chains in their cell walls, enabling larger molecules to penetrate the bacterial cell wall and produce mutations. With the exception ofTA102, all strains have reduced capability to repair DNA-damage which increases the likelihood that such damage results in mutations.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Test Substances [µg per plate] S9 mix
Solvent Control 0 -/+
Test Item 50 -/+
160 -/+
500 -/+
1600 -/+
5000 -/+
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- mitomycin C
- other: 4-N PDA ( 4-Nitro-o-phenylenediamine), 2-AA (Anthracene-2-amine)
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- other: Substance precipitation occurred at the dose of 5000 µg per plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Macrolex Rot E2G, further described as test item in this report, was investigated for point mutagenic effects in the Salmonella/ microsome test (plate incorporation test). The test item, dissolved in DMSO, was administered in doses of up to and including 5000 µg per plate without and with S9 mix on the five Salmonella typhimurium LT2 mutant strains TA1535, TAl00, TA1537, TA98 and TA102, according to the OECD guideline 471. No bacteriotoxic effects were observed. Substance precipitation occurred at the dose of 5000 µg per plate.
The employed positive controls had a marked mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding solvent controls.
No evidence of mutagenic effects were seen in Salmonella typhimurium TA1535 in the absence or presence of S9 mix. Evidence of mutagenic activity of the test item was seen for Salmonella typhimurium TAl00, TA1537 TA98 and TA102. A biologically relevant increase was found in the mutant count compared to the corresponding solvent control in the presence of S9 mix. Positive response started strain specifically at 50 µg/plate.
Therefore, the test item was considered to be mutagenic to Salmonella typhimurium TA100, TA1537 TA98 and TA102 in the presence of S9 mix in the plate incorporation of the Salmonella/microsome test.
Due to these clear positive effects in the plate incorporation test, an independent repeat using the preincubation modification was relinquished.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: positive with metabolic activation
- Executive summary:
Macrolex Rot E2G (CAS no 6829-22-7) was investigated for point mutagenic effects in the Salmonella/microsome test (plate incorporation test). The test item, dissolved in DMSO, was administered in doses of up to and including 5000 µg per plate without and with S9 mix on the five Salmonella typhimurium LT2 mutant strains TA1535, TA100, TA1537, TA98 and TA102, according to the OECD guideline 471.
No bacteriotoxic effects were observed. Substance precipitation occurred at the dose of 5000 µg per plate.
The employed positive controls had a marked mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding solvent controls.
No evidence of mutagenic effects were seen in Salmonella typhimurium TA1535 in the absence or presence of S9 mix. Evidence of mutagenic activity of the test item was seen for Salmonella typhimurium TAl00, TA1537 TA98 and TA102. A biologically relevant increase was found in the mutant count compared to the corresponding solvent control in the presence of S9 mix. Positive response started strain specifically at 50 µg/plate.
Therefore, the test item was considered to be mutagenic to Salmonella typhimurium TA100, TA1537 TA98 and TA102 in the presence of S9 mix in the plate incorporation of the Salmonella/microsome test.
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