Reaction mass of 4,8-diamino-2-[4-(2-ethoxyethoxy)phenyl]-1,5-dihydroxy-9,10-anthraquinone and 4,8-diamino-2-(4-ethoxyphenyl)-1,5-dihydroxy-9,10-anthraquinone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of 1,5-dihydroxy-4-nitro-8-(phenylamino)anthraquinone and 1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone

EC Number:

944-623-4

Molecular formula:

C20 H12 N2 O6

IUPAC Name:

Reaction mass of 1,5-dihydroxy-4-nitro-8-(phenylamino)anthraquinone and 1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

None

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

21 °C

pH:

7.6 - 7.8

Dissolved oxygen:

97 - 99 % at the end of the exposure

Nominal and measured concentrations:

0.1, 1, 10 and 100 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

One out of 10 Daphnia at the concentration of 10 mg/L was found dead at the end of 48 hour exposure period. Apart from this, no mortality was observed at any other cocentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups.

Results with reference substance (positive control):

None

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

48h EC50 of FAT 92504/A to Daphnids was determined to be >100 mg/L

Executive summary:

The short term toxicity of FAT 92504/A to aquatic invertebrates was evaluated in a study conducted according to OECD Guideline 202. In this study, groups of 10 daphnids were exposed to the test concentrations of 0.1, 1, 10 and 100 mg/L for 48 hours. The daphnids were also exposed to blank and vehicle groups. The observations of mobility were recorded to determine the toxicity of the substance. One out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48 hour exposure period. Apart from this, no mortality was observed at any other concentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups. Hence, the 48h EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.