Amidation products of C16-18 (even numbered), C18 unsaturated fatty acids esters with 1,1'-iminodipropan-2-ol

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 June 2016 to 08 December 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

European Community (EC), EC no. 260/2014, Part A: Methods for the Determination of Physico-Chemical Properties, Guideline A.6: “Water Solubility”, Official Journal of the European Union no. L81, March 19, 2014.

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

Organization for Economic Co-operation and Development (OECD), OECD Guidelines for the Testing of Chemicals no. 105: "Water Solubility", July 27, 1995.

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Version / remarks:

United States Environmental Protection Agency (EPA), Product Properties Test Guidelines no. OPPTS 830.7840: "Water Solubility: Column Elution Method; Shake Flask Method", March 1998.

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

column elution method

Specific details on test material used for the study:

Test item 207258/AIdentification MLA-3202Appearance Clear amber-red liquidBatch RC-1045Purity/Composition UVCBTest item storage At room temperatureStable under storage conditions until 17 February 2019 (expiry date)

Key result

Water solubility:

0.54 mg/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

> 7.7 - < 7.8

Details on results:

Preliminary testIt was observed that the test item formed a jelly-like substance, floating freely in the bulk of the water phase. It was not possible to take a sample from the clear water phase without taking also undissolved test item. In order to obtain a sample without undissolved test item filtration and centrifugation were tested. Filtration failed since the test item adsorbs to the 0.2 μm FP 30/0.2 CA-S filter used. Centrifugation failed since concentration decreased with the number of centrifugation steps (5 minutes at 12000 rpm, 20 °C). Based on this it was concluded that the flask method is not applicable for determination of the water solubility of MLA-3202.Main studyThe coefficient of variation (CV) (n = 10) was ≤ 30% at both flow rates. It demonstrated that the system was equilibrated at all flow rates.The maximum difference (MD) on the mean values of the samples at both flow rates was also ≤ 30%. Based on this, the water solubility of the test item is given as the mean value of these measurements and in the conclusion rounded to two significant digits.The turbidity of the eluates was < 0.2 NTU. According to this, no undissolved particles were detected in the eluates.No test item was detected in the samples from the blank column.

 Main Study – Water Solubility of the Test Item

Flow rate [mL/h]	Sample no.	Concentration analysed					pH
		Individual [μg/L]	Mean^1 [μg/L]	CV [%]	Mean^2 [μg/L]	MD [%]
24	1 2 3 4 5 6 7 8 9 10	514 501 522 485 476 501 516 534 521 548	512	4.2	536	9.1	7.8
12	1 2 3 4 5 6 7 8 9 10	645 618 608 592 578 563 528 508 486 475	560	10			7.7

¹Mean of the concentrations obtained at one flow rate.

²Mean of the concentrations obtained at both flow rates.

Conclusions:

The water solubility of the test item at 20°C was 0.54 mg/L.

Executive summary:

The column elution method was applied for the determination of the water solubility of MLA-3202.

 

The study was performed in accordance with the following guidelines:

European Community (EC), EC no. 260/2014, Part A: Methods for the Determination of Physico-Chemical Properties, Guideline A.6: “Water Solubility”, Official Journal of the European Union no. L81, March 19, 2014.

Organization for Economic Co-operation and Development (OECD), OECD Guidelines for the Testing of Chemicals no. 105: "Water Solubility", July 27, 1995.

United States Environmental Protection Agency (EPA), Product Properties Test Guidelines no. OPPTS 830.7840: "Water Solubility: Column Elution Method; Shake Flask Method", March 1998.

 

The water solubility of the test item at 20 °C was 0.54 mg/L.

The pH of the aqueous samples was 7.7 – 7.8.

Description of key information

Key value determined in a GLP accredited laboratory study in accordance with OECD Guideline 105, EU Method A6 & US EPA Procedure OPPTS 830.7840.

Key value for chemical safety assessment

Water solubility:

0.54 mg/L

at the temperature of:

20 °C

Additional information

The column elution method was applied for the determination of the water solubility of MLA-3202.

 

The water solubility of the test item at 20 °C was 0.54 mg/L.

The pH of the aqueous samples was 7.7 – 7.8.