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EC number: 681-644-5 | CAS number: 136369-04-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- The temperature for the main study lay in a range of 23.0 to 24.8 °C (test item and positive control). As no significant immobility occurred during the test, this deviation was stated as uncritical.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- potassium 5-amino-1H-1,2,3,4-tetrazol-1-ide
- Cas Number:
- 136369-04-5
- Molecular formula:
- CH2KN5
- IUPAC Name:
- potassium 5-amino-1H-1,2,3,4-tetrazol-1-ide
- Test material form:
- solid: particulate/powder
- Details on test material:
- Batch no.: 08KI030
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The holding is performed similar to the method described in EN ISO 6341, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), edition 9, adopted 27. Apr. 2007.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Resulting hardness in mmol/L: 2.50
Resulting hardness in mg CaCO3/L: 250 - Test temperature:
- 23 - 24.8 °C
- pH:
- 7.7 +- 0.2
- Dissolved oxygen:
- 7.3 - 8.6 mg/L
- Nominal and measured concentrations:
- Nominal: 1.0 / 2.2 / 4.6 / 10 / 22 / 46 / 100 mg/L.
The measured concentrations showed very good correlation with the nominal concentrations. The recovery rate after 48 hours was in a range of 97 % - 101 % of the start concentration. The measured concentrations were in a range of 100 % - 103 % of the nominal concentration. Therefore the determination of the biological results was based on the nominal concentration. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (0.5 / 0.625 / 1.0 / 1.25 / 1.5 / 2.0 / 2.5 / 3.0 mg/L)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: EC50i
- Effect conc.:
- 49 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: EC50i
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Results with reference substance (positive control):
- The 24h-EC50i of potassium dichromate was determined as 1.6 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All validity criteria were met. For the estimation of the EC50s of test item and positive control, the fits showed sufficient statistical correspondence of the data with the doseresponse- equation. The 24h-EC50i of potassium dichromate was determined as 1.6 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L. The analytical determinations of the test item showed very good correlation between nominal and measured concentrations and very good recovery rates. Therefore, the test item 5-Aminotetrazol Potassium can be stated as stable under the test conditions and the determination of the results was based on the nominal concentrations. The result of the test can be considered valid. The following results were determined for the test item 5-Aminotetrazol Potassium (species: Daphnia magna): 24h-NOEC = 10 mg/L, 48h-NOEC = 4.6 mg/L, 24h-EC50i = 49 mg/L, 48h-EC50i = 13 mg/L.
- Executive summary:
The main study was performed using seven concentrations ranging from 1 to 100 mg/L. Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. Four concentrations showed toxicity between 55 and 90% immobilisation. Two of the animals (10 %) were immobilised in the control. The 24h-EC50i of potassium dichromate was tested in a current reference test. The value was determined as 1.6 mg/L, lying within the demanded range of 0.6 – 2.1 mg/L. At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC. The recovery after 48 hours ranged from 97 to 102 % of the start concentration. The correlation between nominal and measured concentration was 100% - 103 % at the beginning and 100 – 102 % at the end. Therefore, the determination of the biological results was based on the nominal concentration. The following results were determined for the test item 5-Aminotetrazol Potassium (species: Daphnia magna): 24h-NOEC = 10 mg/L, 48h-NOEC = 4.6 mg/L, 24h-EC50i = 49 mg/L, 48h-EC50i = 13 mg/L.
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