Chromium oxide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

26 September - 20 November 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

and EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

Temperature lay in a range between 20.5 and 21.8°C, and was thus lower than stated in the guideline. However, no immobility occurred and the deviation is stated as uncritical

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Rheinland-Pfalz

Test material

Test material information

Constituent 1

Reference substance name:

Chromium triacetate

EC Number:

213-909-4

EC Name:

Chromium triacetate

Cas Number:

1066-30-4

IUPAC Name:

chromium(3+) triacetate

Details on test material:

- Name of test material (as cited in study report): Chromium (III) acetate
- Substance type: yellow-greenish powder
- Physical state: solid
- Analytical purity: > 95%
- Lot/batch No.: 1000141379
- Storage condition of test material: room temperature, 20 ± 5°C, dry, in closed vessel

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): After 75 h precipitation was observed and a test medium renewal was introduced

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Dehner Garten Center, Neustadt, Germany
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation (length definition, mean, range and SD): 2 ± 1 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period: 04 September - 26 September 2008
- Type and amount of food: warmwater fishfood and daphnia, totalling to about 1-2% of body weight per day
- Feeding frequency: three times a day
- Health during acclimation (any mortality observed): no

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

2.02 mmol/L

Test temperature:

20.8 - 23.8 °C

pH:

6.0 ± 0.1 (adjusted every 12 hours)

Dissolved oxygen:

> 7.7 mg/L (93%)

Nominal and measured concentrations:

100 mg/L (nominal); 87.8 mg/L (measured, time-weighed mean)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass aquaria, maximal volume 10 L
- Aeration: yes, accomplished with glass tubes, frequency of bubbles 1/s
- Renewal rate of test solution (frequency/flow rate): once, after 75 h, following observation of precipitation
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate:1 fish/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water
- Total organic carbon: < 1.0 mg/L
- Metals:
- Pesticides: < limit of detection
- Chlorine: 26 mg/L
- Ca: 64 mg/L
- Conductivity at 20°C: 393 µS/cm
- Intervals of water quality measurement: 12 h


OTHER TEST CONDITIONS
- Adjustment of pH: yes, 6.0 ± 0.1
- Photoperiod: 12/12 hours light / dark
- Light intensity: neon tubes


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, immobility

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

24 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 7

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 8

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 9

Duration:

72 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 10

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 11

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 12

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: precipitation occurred after 72 h and a renewal of the medium was introduced. Differences between nominal and measured values are within a 20% range

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table: pH values

Nominal concentration in mg/L	pH at t in h (old/new test solution)
	0 h	12 h		24 h		36 h		48 h		60 h		72 h		96 h
	new	old	new	old	new	old	new	old	new	old	new	old	new	old
0	6.0	6.4	6.0	7.2	6.0	7.4	6.0	6.8	6.0	6.4	6.0	6.6	6.0	7.3
100	6.0	6.4	6.0	6.8	6.0	6.9	6.0	6.6	6.0	6.4	6.0	5.8	5.8	6.5

Table: Temperature in °C

0 h	24 h	48 h	72 h	96 h
20.8	22.9	23.6	23.8	23.8

Table: Measured concentrations

Nominal concentration in mg/L	Time in hours	Measured concentration in mg/L	% Recovery	% of Nominal concentration
0	0	LOQ	-	-
100	0	88.1	-	88
0	24	LOQ	-	-
100	24	96.7	110	97
0	48	LOQ	-	-
100	48	96.7	100	97
0	72	LOQ	-	-
100	72	69.5	72	70
0	75	LOQ old solution	-	-
100	75	83.4 old solution	120	83
0	75	LOQ new solution	-	-
100	75	88.7 new solution	106	89
0	96	LOQ	-	-
100	96	76.1	86	76

Table: Time-weighed-mean of the measured concentrations in mg/L

Nominal concentration in mg/L	Experimental period	Duration of period [d]	Measured concentration in mg/L		Log (Conc.)		Summand
			Start	End	Start	End
100	1	1	88.05	96.67	1.945	1.985	3.930
	2	1	96.67	96.67	1.985	1.985	3.971
	3	1	96.67	69.50	1.985	1.842	3.827
	4	0.125	69.50	83.41	1.842	1.921	0.470
	5	0.875	88.72	76.13	1.948	1.882	3.351
	Total	4					15.549
	Geometric Mean in mg/L						87.83