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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
not specified
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Octanoic acid, compound with octylamine (1:1)
EC Number:
241-477-7
EC Name:
Octanoic acid, compound with octylamine (1:1)
Cas Number:
17463-34-2
Molecular formula:
C8H19N.C8H16O2
IUPAC Name:
octan-1-amine; octanoic acid
Details on test material:
- Analytical purity: 100%
- CAS-No: 17463-34-2
Specific details on test material used for the study:
Carbon content calculated from sum formula: 703 mg/g
calculated COD: 2.69x10^3 mg/g (O2)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant in Frankfurt-Sindlingen
- Pretreatment: stirred in mineral medium for 5 days
- Concentration of sludge: 22 mg/l dry weight
Duration of test (contact time):
14 d
Initial test substance concentration
Initial conc.:
131 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The substance was weighted directly into the test vessel.

TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20 ± 1 °C

TEST SYSTEM
- Culturing apparatus: BOD vessels

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
62
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
68
Sampling time:
14 d
Details on results:
In a toxicity test no derogation of degradation occured at the test concentration.

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable
Conclusions:
The test substance is readily biodegradable after 14 days.
Executive summary:

The ready biodegradability of the test item was tested in a Manometric Respirometry Test with nonadapted inoculum over a period of 14 days similar to OECD Guideline for Testing of Chemicals 301 F. The test item in the concentration of 131 mg/l reached a degree of degradation of 68% after 14 days, respectively. The test item must be regarded as readily biodegradable after 14 days.