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EC number: 255-965-2 | CAS number: 42844-93-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2017-05-06 to 2017-05-12 with the definitive exposure period from 2017-05-08 to 2017-05-12 at the test facility.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- According to OECD and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- [1,3-dihydro-5,6-bis[[(2-hydroxy-1-naphthyl)methylene]amino]-2H-benzimidazol-2-onato(2-)-N5,N6,O5,O6]nickel
- EC Number:
- 255-965-2
- EC Name:
- [1,3-dihydro-5,6-bis[[(2-hydroxy-1-naphthyl)methylene]amino]-2H-benzimidazol-2-onato(2-)-N5,N6,O5,O6]nickel
- Cas Number:
- 42844-93-9
- Molecular formula:
- C29H18N4NiO3
- IUPAC Name:
- Nickel, [1,3-dihydro-5,6-bis[[[2-(hydroxy-kO)-1-naphthalenyl]methylene]amino-kN]-2H-benzimidazol-2-onato(2-)]-, (SP-4-2)-
- Test material form:
- solid: nanoform
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test item
C.I. Pigment Orange 68
Preparation of the saturated solution
The saturated solution (1.00 mg/L test item was weighed out) was prepared with dilution water two days prior to the start of the exposure (0 hours).
Treatment
The test item was dispersed in the dilution water by stirring with approximately 1100 rpm for 48 hours at room temperature. After completion of stirring, the test item dispersion was filtered with a membrane filter (0.45 µm, RC, MACHEREY-NAGEL) to achieve the saturated solution. This procedure was repeated for every water renewal.
Test concentration
The saturated solution was tested in a limit test. The selection of the test concentration is based on the derivation of a threshold concentration (TC) from the results of an algae toxicity test and a Daphnia acute toxicity test, both EC50 > saturated solution prepared with a nominal loading of 1.00 mg test item/L
(algae: SCHEERBAUM, 2015; daphnia: NOACK, 2015).
Control
7 fish in dilution water (without test item) were tested under the same test conditions as the test replicates.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: All fish used in the test were gained at NOACK LABORATORIEN from a single brood stock. (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin)
- Length at study end (length definition, mean, range and SD): Average body length: 2.37 cm
- Weight at study end (mean and range, SD): Average body weight: 0.12 g
- Method of breeding:Holding was performed at the test facility at 23 +/- 2 °C and diffuse light (0.1 - 10 µmol photons • m-2 • s-1, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value. No disease treatments were administered throughout holding and testing. Food was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day. The test fish were not fed 24 h before the test started.
- Feeding during test: No feeding during test
ACCLIMATION
- Acclimation period: Zebrafish with at least 12 days of acclimatisation and mortality < 5 % within these days before the study starts were used in the test.
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: 4 % of the fish body weight per feeding day. The test fish were not fed 24 h before the test started. Food: Sera Vipan; SERA GMBH, D-52518 Heinsberg
- Feeding frequency: 3 times per week.
- Health during acclimation (any mortality observed): No mortality observed, no disease treatments were administered throughout holding and testing.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Total Hardness at day 0: 59 mg CaCO3/L
- Test temperature:
- Please refer to "Any other information on materials and methods"
- pH:
- Please refer to "Any other information on materials and methods"
- Dissolved oxygen:
- Please refer to "Any other information on materials and methods"
- Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable):
- Material, size, headspace, fill volume: Glass aquaria of 3 L were used, (dimensions: 11.5/13/20 cm), 2.5 L test volume
- Aeration: Gentle aeration was provided
- Renewal rate of test solution (frequency/flow rate): A semi-static test with one water renewal of the test media after 48 hours was performed.
- Control: 7 fish in dilution water (without test item) were tested under the same test conditions as the test replicates
- Reference item: No reference item is recommended for this test according to the guideline.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control and pH-control (replicates): 1
- Biomass loading rate: 0.336 g fish per L test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove possible chlorine.
Nominal water parameters:
Total hardness: 10 - 250 mg CaCO3/L
pH-value: 6.0 - 8.5
Acidity: 0.1 mmol/L
(recent measurement 2017-04-26)
Acid capacity: 0.6 mmol/L
(recent measurement 2017-04-26)
Conductivity: 151 µS/cm
(recent measurement 2017-04-26)
- Culture medium different from test medium: no
- Intervals of water quality measurement:The pH-value, temperature and oxygen saturation was measured in all test vessels at test start and end and every
24 hours and from fresh and old test media after renewal. Total hardness was determined at the start of the exposure out of the control. During the test the water temperature was recorded continuously (once per hour) with a data logger. The light intensity on the surface of the test aquaria was measured at the start of the exposure.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: A photoperiod of 16 h light / 8 h dark corresponding to natural daylight hours occurred during the course of the study.
- Light intensity: 7 - 750 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Fish were considered dead if there was no visible movement (e.g. gill covers movement) and if touching of the caudal peduncle produced no reaction. Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.). Observations were made after 2, 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Range finding study: None - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: Not observed
- Mortality of control: 0%
- Other adverse effects control: Not observed
- Abnormal responses: Not observed
- Any observations (e.g. precipitation): - Results with reference substance (positive control):
- No reference substance tested
- Reported statistics and error estimates:
- Evaluation
The LC0 and LC100 after 96 h was derived from the raw data of the limit concentration. LC50-values do not have to be calculated in a limit test.
Software
The data for the tables in this report were computer generated and have been rounded for presentation from the full derived data. Consequently if calculated manually based on the given data minor variations may occur from these figures. The following software was used for calculations:
Excel, MICROSOFT CORPORATION
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Biological Data
All effect levels are given based on the nominal threshold concentration of the test item after 96 hours.The LC0after 96 hours was ≥ 1.00 mg/L..
Observations of Sub Lethal Effects and Normal Behaviour in the Test Vessels
Saturated solution with a nominal loading of
[mg/L]Effect *
Number of fish effected at observation time [hours]
2
24
48
72
96
1.00
(1)
7
7
7
7
7
Control
(1)
7
7
7
7
7
*) The numbers in brackets correspond to the following observations:
(1) = Normal behaviour
Cumulative Mortality [%] in the Test Vessels
Saturated solution with a nominal loading of
[mg/L]Cumulative Mortality [%] after different exposure periods [hours]
2
24
48
72
96
1.00
0
0
0
0
0
Control
0
0
0
0
0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All effect levels are given based on the saturated solution with a nominal loading of 1 mg test item/L. The saturated solution of the test item found to have no effect on the vitality of zebrafish. In conclusion, the LC0 corresponds to the saturated solution with a nominal loading 1.00 mg test item/L.
- Executive summary:
The acute toxicity of the test item (batch no.DEB2188394) to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203 (1992) and EU Council Regulation (EC) No. 440/2008/ Method C.1 (2008) from 2017-05-06 to 2017-05-12 with the definitive exposure period from 2017-05-08 to 2017-05-12 at the test facility.
An acute toxicity test with the saturated solution (nominal loading of 1.00 mg/L) was conducted under semi-static conditions with one water renewal of the test media after 48 hours of exposure. Duration of the test was 96 hours. Seven test organisms were exposed to the threshold concentration and the control. Water quality parameters (temperature, pH-value and oxygen-saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits.
No analytical monitoring will be carried out, since no suitable method for the determination of thetest item could be established. No suitable column was found which is compatible for liquid chromatography.
All validity criteria of the test guideline were met.
LC-Values (2 – 96 hours) of the Test Item
Based on the saturated solution with a nominal loading of 1 mg/L
LC100=
Lowest test item concentration with
100 % mortality after 96 h> 1.00 mg/L
LC0=
Highest test item concentration with
0 % mortality after 96 h1.00 mg/L
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