Phosphorous ester

Endpoint summary

Additional information

Short-term toxicity to fish

The purpose of this study was to evaluate the acute toxicity of the test item GARDO TP 10451 to fish. Young fish were exposed in a static test to the test item for 96 hours, added to test water at a range of concentrations. A limit test was performed with a nominal concentration of 100 mg test item/L. As agreed with the Sponso, analytical measurements of the test item concentrations were not performed during this study because the calculative highest solubility of GARDO TP 10451 is below the lowest level of quantification

(1.1734 ng/L). Therefore, all reported biological results are related to the nominal concentrations. The LC50 was determined to be higher than 100 mg/L.

Short-term toxicity to aquatic invertebrates

The purpose of this study was to evaluate the influence of the test item GARDO TP 10451 on the mobility respectively survival of Daphnia magna. Young Daphnia were exposed in a static test to the test item for 48 hours, added to test water at a range of concentrations. The nominal test item concentrations were: 6.3; 12.5; 25; 50 and 100 mg/L. Agreed with the Sponsor analytical measurements of the test item concentrations were not performed during this study because the calculative highest solubility of GARDO TP 10451 is below the lowest level of quantification (1.1734 ng/L). Therefore, all reported biological results are related to the nominal concentrations. The EC50 was determined to be higher than 100 mg/L.

Toxicity to aquatic algae and cyanobacteria

The purpose of this study was to determine the effect of the test item GARDO TP 10451 on the growth of an unicellular green algal species Pseudokirchneriella subcapitata (formerly: Selenastrum capricornutum). Exponentially growing cultures of Pseudokirchneriella subcapitata were exposed to various concentrations of the test item over several generations under defined conditions. The algal growth in relation to a control culture was determined over a fixed test period of 72 hours and thus, over several algal generations. The test method of application and the test species Pseudokirchneriella subcapitata are recommended by the test guidelines. The nominal test item concentrations were: 6.25; 12.5; 25; 50 and 100 mg/L. Agreed with the Sponsor analytical measurements of the test item concentrations were not performed during this study because the calculative highest solubility of GARDO TP 10451 is below the lowest level of quantification (1.1734 ng/L). Therefore, all reported biological results are related to the nominal concentrations. The 72 -h EC50 based on growth rate was determined as 105.64 mg/L. The overall NOEC was determined to be 12.5 mg/L.

Toxicity to microorganisms

The influence of GARDO TP 10451 on the activity of activated sludge was tested in a 3 hours respiration inhibition test (08/625 -027AS). Nominal test concentrations of 10, 31, 100, 313 and 1000 mg/L were chosen to permit the determination of the EC50 value. During incubation the temperature was around 19.7 -20 .6 °C. Based on measured inhibition rates it can be stated that the 3 -hour EC20, EC50 and EC80 values were higher than 1000 mg/L. The study was conducted according to the OECD guideline 209 and the EU-Guideline C.11.