2-[2-(3,4-dihydro-6-methoxy-1(2H)-naphthylidene)ethyl]-2-ethylcyclopentane-1,3-dione

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guidelines and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-[2-(3,4-dihydro-6-methoxy-1(2H)-naphthylidene)ethyl]-2-ethylcyclopentane-1,3-dione

EC Number:

212-705-2

EC Name:

2-[2-(3,4-dihydro-6-methoxy-1(2H)-naphthylidene)ethyl]-2-ethylcyclopentane-1,3-dione

Cas Number:

850-92-0

Molecular formula:

C20H24O3

IUPAC Name:

2-ethyl-2-[2-(6-methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethyl]cyclopentane-1,3-dione

Test material form:

solid: crystalline

Details on test material:

Test item: Ethyl-secodion
CAS No.: 850-92-0
Batch No.: L49030N
Physical state: Solid, crystalline powder
Colour: Beige
Storage: At room temperature, dry place

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Type of inoculum: aerobic mixed bacterial culture
Source: Municipal wastewater treatment plant, Esztergom-Kertváros
Preparation of inoculum test culture:
250.0 ml inoculum test culture with 1.0 g/L dry substance was made by mixing 137 ml of stock culture with 113 ml mineral medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

10.1 - 10.22 other: mg/250ml

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Following test vessels were prepared and analysed for the study:
- Test item (in two replicates)
- Blank control (in two replicates)
- Reference solution (positive control)
- Reference and test item solution (toxicity control)
- Sterilized solution of test item (abiotic control).
The test vessels were tightly closed with measuring heads and placed into thermostat. The temperature during the test was adjusted to 21 ± 1 °C. The vessels were kept in complete darkness and they were stirred continuously.
The vessels were kept in thermostat for 28 days.

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

30

Sampling time:

28 d

Remarks on result:

other: mean of two replicates

Details on results:

Reference solution (positive control): 90%
Reference and test item solution (toxicity control): 45%
Sterilized solution of test item (abiotic control): 0%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

In this biodegradability of "Ethyl-secodione" (CAS No. 850-92-0) the results indicated that the test item is not ready biodegradable in the aquatic environment.