2-hydroxyethyl methacrylate

Immunotoxicity

Administrative data

Endpoint:

immunotoxicity, other

Remarks:

in-vitro

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

HEMA Enhances IgG1 Production by Human B-cells in vitro.

Author:

Andersson J. ad Dahlgren U.

Year:

2010

Bibliographic source:

J Dent Res 89(12):1461-1464

Materials and methods

Principles of method if other than guideline:

The in vitro production of IgG1, IgM, and IgA in supernatants from purified human CD19+
B-lymphocyte cultures, containing different concentrations of HEMA, was assayed with ELISA.
Proliferation was measured by [methyl-3H] thymidine incorporation.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxyethyl methacrylate

EC Number:

212-782-2

EC Name:

2-hydroxyethyl methacrylate

Cas Number:

868-77-9

Molecular formula:

C6H10O3

IUPAC Name:

2-hydroxyethyl methacrylate

Test material form:

liquid

Results and discussion

Any other information on results incl. tables

Of the different HEMA concentrations used, the lower concentrations of 15 and 37.5 µmol/L caused a significant increase in IgG1 production, but not in IgM or IgA production, in vitro. The lower HEMA concentrations did not significantly change B-cell proliferation. When B-cells were exposed to 75 μmol/L or 150 μmol/L HEMA, no significant changes in IgG1 were demonstrated. At the highest concentration of 750 μmol/L, HEMA significantly suppressed IgG1 and IgM production, as well as B-cell proliferation, in vitro.

 

 

Applicant's summary and conclusion

Conclusions:

In conclusion, HEMA can, at certain concentrations, selectively enhance human B-lymphocyte IgG1 production.