Registration Dossier
Registration Dossier
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EC number: 233-032-0 | CAS number: 10024-97-2
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, limited no. of strains used
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of inhalation anaesthetics: trichloroethylene, divinyl ether, nitrous oxide and cyclopropane
- Author:
- Baden J.M., Kelley M., Mazze R.I., & Simmon, V.F.
- Year:
- 1 979
- Bibliographic source:
- Br. J. Anaesth. 51; pp 417-421
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- limited no. of strains used
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dinitrogen oxide
- EC Number:
- 233-032-0
- EC Name:
- Dinitrogen oxide
- Cas Number:
- 10024-97-2
- Molecular formula:
- N2O
- IUPAC Name:
- Dinitrogen Oxide
- Test material form:
- gas under pressure: liquefied gas
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254
- Test concentrations with justification for top dose:
- 0, 1, 3, 9, 27, 81%
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: vinylidene chloride, 2-anthramine
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No increase in the number of revertant colonies were observed in strain TA100 or TA1535. At the maximum dose tested, 81%, no evidence of cytotoxicity was observed.
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