Anisole

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

no data

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Lack of information regarding test conditions, and specially for concentrations and open or closed system.

Qualifier:

according to guideline

Guideline:

other:

Deviations:

not applicable

Principles of method if other than guideline:

other: DIN L15 Fischtest "Bestimmung der Wirkung von Wasserinhaltsstoffen auf Fische"

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

Test fish :
- Age : Information not available.
- Length : 5-7 cm.
- Weight : 1.5+/-0.3 g.
- Loading : 1.5+/-0.3 g/litre of test solution.
- Pretreatment : Information not available.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

15+/-3°d

pH:

7-8

Details on test conditions:

Test conditions :
- Dilution water source : Drinking water.
- Dilution water chemistry : Hardness 15+/-3°d; pH: 7-8 Free from chlorine; Mg, Ca concentration 2.7+/-0.5 mmol/L; Proportion Ca:Mg ions 4:1;
Acid capacity KS 4.3 0.7-0.9 mmol/L; Aeration.
- Stock and test solution and how they are prepared : Information not available.
- Concentrations dosing rate, flow-through rate, in what medium : Test substance was diluted in drinking water. No information available about test
concentrations.
- Vehicle/solvent and concentrations : Information not available.
- Stability of the test chemical solutions : Information not available.
- Exposure vessel type : Aquarium (height: 24 cm; length: 32 cm; depth: 22 cm; volume: 17 L); 10 L of test solution and 10 fish per aquarium; Test solution mixed by aeration. It was not mentioned if the aquariums were closed or not during the test period.
- Number of replicates, fish per replicate : 10 fish per replicate. No information available about number of replicates.
- Water chemistry in test (DO, pH) in the control and one concentration where effects were observed : In test, the O2-concentration of test solution was 5 mg/L. No information available about pH.
- Control group : Information not available
- Test temperature range : 20+/-1°C.
- Method of calculating mean measured concentrations : The EC50-value was evaluated by graphic exploitation.

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

120 - 149 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Nominal concentrations : Information not available.
- Measured concentrations : Information not available.
- Element value : This study reports the results obtained with Leuciscus idus melanotus under comparable conditions in two different laboratories : "Institut für Wasser-, Boden- und Lufthygiene des Bundesgesundheitsamtes Berlin" (JUHNKE I.) and "Landesanstalt für Wasser und Abfall NW" (LÜDEMANN D.).
Results obtained by JUHNKE I. :
LC0 (48 h) = 79 mg/L
LC50 (48 h) = 120 mg/L
LC100 (48 h) = 139 mg/L
Results obtained by LÜDEMANN D. :
LC0 (48 h) = 99 mg/L
LC50 (48 h) = 149 mg/L
LC100 (48 h) = 198 mg/L
- Statistical results : Information not available

Validity criteria fulfilled:

not specified

Conclusions:

Anisole is not harmful to fish.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Publication in german. No summary available. Static test.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Leuciscus idus

Test type:

static

Water media type:

freshwater

pH:

7.5 +/- 0.3 (adjusted if necessary)

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

No more information.

Validity criteria fulfilled:

not specified

Conclusions:

Anisole is not harmful to fish.

Description of key information

LC50 > 100 mg/L 

Additional information

Two data are available for this endpoint. Due to lack of information, these data are scored 3 in the endpoint study summary. However, both studies are consistent in their results, concluding that anisole is not harmful to fish. Therefore, a weight of evidence approach is performed to document this endpoint.