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EC number: 258-469-4 | CAS number: 53306-54-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Version / remarks:
- July 2006
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis(2-propylheptyl) phthalate
- EC Number:
- 258-469-4
- EC Name:
- Bis(2-propylheptyl) phthalate
- Cas Number:
- 53306-54-0
- Molecular formula:
- C28H46O4
- IUPAC Name:
- 1,2-bis(2-propylheptyl) benzene-1,2-dicarboxylate
- Details on test material:
- - Name of test material (as cited in study report): Palatinol 10-P
- Analytical purity: 98.3 area-% (GC, DB-1), 98.8 area-% (GC, DB-5 HT), see report No 17L00129
- Homogeneity: given
- Lot/batch No.: F151_2017
- Expiration date of the lot/batch: 07 Dec 2018
1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- The control and test item treatments were applied once at the beginning of the study. The respective test item amount was weighed out for each test item concentration on quartz sand (1% of the entire soil amount per concentration) and blended thoroughly with quartz sand. Afterwards the test item - quartz sand mixture and an appropriate amount of demineralised water (adjusting the soil to a moisture of 50 % and 45 % of the maximum water holding capacity (MWHC), respectively) were added to the soil. Subsequently, the soil was mixed carefully with a mixer (3 min) to ensure a homogeneous distribution.
The treated soil was transferred to the test container and the seeds were sown.
Test organisms
open allclose all
- Species:
- Avena sativa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- KWS Contender
Origin: KWS Lochow GmbH, Ferdinand-von-Lochow-Straße 5, 29303 Bergen, Germany
- Species:
- Allium cepa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- Exhibition
Origin: Hild Samen GmbH, Postfach 1161, 71666 Marbach, Germany
- Species:
- Beta vulgaris
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Finola KWS3
Origin: KWS Saat SE, Grimsehlstraße 31, 37574 Einbeck, Germany
- Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Sherlock
Origin: KWS Saat SE, Grimsehlstraße 31, 37574 Einbeck, Germany
- Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Mafalda
Origin: Hild Samen GmbH, Postfach 1161, 71666 Marbach, Germany
- Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Obelix
Origin: Delley Samen und Pflanzen AG, Schloss Delley 40, route de Portalban, CH-1567 Delley, Switzerland
- Species:
- other: Allium tuberosum
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- Shiva
Origin: Hild Samen GmbH, Postfach 1161, 71666 Marbach, Germany
Study design
- Test type:
- seedling emergence and seedling growth test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Remarks:
- LUFA 2.2
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks:
- In the 2nd definitive study the test for salad, onion and garlic chives was conducted over a period of 28 days.
Test conditions
- Test temperature:
- 1st definitive study - actual: 18.6 - 23.7 °C
2nd definitive study - actual: 17.3 - 25.3 °C - Moisture:
- 1st definitive study - actual: 46.4 - 98.8% relative humidity
2nd definitive study - actual: 44.6 - 97.4% relative humidity - Details on test conditions:
- Sowing: Test containers were filled each with the same amount of treated soil and seeding holes were made with a seedling pistil. One seed was given into each hole and covered with soil. During cultivation the test containers were bottom watered and fertilized as necessary.
Number of seeds:
Oats, onion, garlic chives, sugar beet, rape, lettuce: 5 seeds per test container, corresponding to 40 seeds per test concentration
Soybean: 3 seeds per test container, corresponding to 24 seeds per test concentration - Nominal and measured concentrations:
- 1000 - 500 - 250 - 125 - 62.5 mg/kg DW + Control
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Key result
- Species:
- Allium cepa
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Key result
- Species:
- other: Allium tuberosum
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Key result
- Species:
- Beta vulgaris
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Lactuca sativa
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Allium cepa
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- other: Allium tuberosum
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Beta vulgaris
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Lactuca sativa
- Duration:
- 28 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Allium cepa
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- other: Allium tuberosum
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Beta vulgaris
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Lactuca sativa
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height, fresh weight, number of emerged seedlings
- Details on results:
- The basis for effect in the different species refers to shoot height, shoot fresh weight as well as to the number of emerged seedlings.
- Reported statistics and error estimates:
- Percentage of inhibition
The percentage of inhibition of biomass growth (shoot height, shoot fresh weight) and rate of emergence were calculated in relation to the control.
All dead plants were excluded from the calculation of shoot height and fresh weight (above-ground biomass).
NOEC/LOEC of biomass growth/emergence
One Way Analysis of Variance (ANOVA) and Dunnett's test were carried out for the determination of statistically significant
differences compared to control replicates. When running a One Way Analysis of Variance, a Normality test and an Equal Variance test were done first. P-values for both, Normality and Equal Variance test, are 0.05. The a-value for ANOVA (acceptable probability of incorrectly concluding that there is a
difference) is a =0.05.
Software
The data for the tables in the report were computer generated and rounded for presentation from the fully derived data.
Consequently, if calculated manually based on the given data, minor deviations may occur from these figures.
Excel, Microsoft Corporation
SigmaPlot (Windows), SPSS Incorporation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Potential toxic effects of the test item were assessed on day 7, 14, 21 and 28 by visual observations (number of emerged seedlings and phytotoxic effects) and at test end by shoot height and shoot fresh weight determination.
No test item related phytotoxic effects and mortality occurred during the test period for all plant species.
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