3,5,5-trimethylhexanoyl chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

3,5,5-trimethylhexanoyl chloride

EC Number:

253-168-4

EC Name:

3,5,5-trimethylhexanoyl chloride

Cas Number:

36727-29-4

Molecular formula:

C9H17ClO

IUPAC Name:

3,5,5-trimethylhexanoyl chloride

Details on test material:

- Name of test material (as cited in study report): Isononansaeurechlorid
- Physical state: liquid
- Analytical purity: 98-99 %

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thome GmbH, Biberach (FRG)
- Age at study initiation: 8 - 9 weeks
- Housing: cages type DK III of Becker, singly
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24 °C
- Humidity (%): 30 - 70 %
- Photoperiod (hrs dark / hrs light): 12 hours

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

1 h

Concentrations:

0.1, 0.24, 0.47 mg/l

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

ca. 0.24 mg/L air

Exp. duration:

1 h

Mortality:

0.1 mg/l: 0/10
0.24 mg/l: 6/10, All male animals and 1 female animals died on day 0.
0.47 mg/l: 10/10, All animals died during days 0 and 1.

Clinical signs:

other: In the low concentration clinical examination revealed irregular and accelerated respiration, restlessness and then apathy during exposure. These symptoms disappeared post exosure on exposure day. The mid concentration additionally resulted in piloerectio

Body weight:

Body weight gain was not affected over the whole post exposure period in the low concentration.

Gross pathology:

Necropsy of the animals that died showed general congestion (all animals) and intensified hyperemia of the lungs.
No macroscopic pathologic findings were noted in the animals killed at the end of the study.

Any other information on results incl. tables

Cumulated lethality on day	Test group concentation
	0.1 mg/l		0.24 mg/l		0.47 mg/l
	Male	Female	Male	Female	Male	female
0	0/5	0/5	5/5	1/5	2/5	0/5
1	-	-	-	-	3/3	5/5
2	-	-	-	-	-	-
7	-	-	-	-	-	-
14	-	-	-	-	-	-
Total at end of the study	0/10		6/10		10/10