Phenethyl phenylacetate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

Experimental data of read across substances

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

no guideline available

Principles of method if other than guideline:

WoE report is based on two long term toxicity to aquatic invertebrates studies as-
WoE 2 and WoE 3.

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

WoE 2- Concentrations: (nominal values) control, vehicle control, - 0.040, 0.097, 0.240, 0.580, 1.40 mg/L ,geometric ratio: 2.4

Vehicle:

yes

Remarks:

WoE 2- Dimethylformamide

Details on test solutions:

WoE 2- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethylformamide
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): constant concentration 100 microL/L

Test organisms (species):

Daphnia magna

Details on test organisms:

WoE 2, WoE 3: Common Name: Water flea

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

WoE 2- 20 ±1 °C

Nominal and measured concentrations:

WoE 2- (nominal values) control, vehicle control, - 0.040, 0.097, 0.240, 0.580, 1.40 mg/L ,geometric ratio: 2.4 But 1.40 mg/L was the adjustable highest concentration of test solution.

Details on test conditions:

WoE 2- TEST SYSTEM
- Type (delete if not applicable): closed, covered water surface with teflon sheet
- Material, size, headspace, fill volume: test solution volume 80 mL/vessel
- Renewal rate of test solution (frequency/flow rate): total amount of test solution every day
- No. of organisms per vessel: 10/concentration (1/vessel)
- No. of vessels per concentration (replicates): 10

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
- Light intensity: less than 800 lux


TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric ratio 2.4
- Test concentrations: 0.040, 0.097, 0.240, 0.580, 1.40 mg/L

Reference substance (positive control):

not specified

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1.18 - < 1.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: EC50 value of test chemical was determined to be > 1.18 to < 1.2 mg/l.

Key result

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1.18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: LOEC value of test chemical was determined to be 1.18 mg/l

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

> 0.17 - < 0.519 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: NOEC value of test chemical was determined to be > 0.17 to < 0.519 mg/l.

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively.

Executive summary:

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

 

The first study of long term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. The duration of study was 21 days. Dimethylformamide was used as a vehicle with a concentration of 100 microL/L. Test concentrations were taken as (nominal values) control, vehicle control of 0.040, 0.097, 0.240, 0.580, 1.40 mg/L in the geometric ratio: 2.4. The adjustable highest concentration of test solution was 1.40 mg/l. Test vessel was of closed, covered water surface with teflon sheet. Test solution volume was taken as 80 mL/vessel. Total amount of test solution was renewed every day. Number of organisms per vessel was taken as 10 per concentration, one test organism per vessel was taken for study. The number of vessels per concentration (replicates) was taken as ten. Photoperiod was given for 16 hours light with 8 hours darkness. Light intensity provided was less than 800 lux. Test conditions includes the temperature of 20 ±1 °C. Analytical monitoring was done by HPLC. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days NOEC, LOEC and EC50 value of test chemical was determined to be 0.519 mg/l, 1.18 mg/l and > 1.18 mg/L respectively. On the basis of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.

 

The second study of long-term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. the duration of study was 21 days. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days EC50 and NOEC value of test chemical was determined to be 1.2 mg/l and 0.71 mg/l respectively. On the basis of the value of NOEC the test chemical was classified as Chronic category 2 as per CLP criteria.  

 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively. Based on the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.  

Description of key information

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

> 0.17 - < 0.519 mg/L

Additional information

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

 

The first study of long term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. The duration of study was 21 days. Dimethylformamide was used as a vehicle with a concentration of 100 microL/L. Test concentrations were taken as (nominal values) control, vehicle control of 0.040, 0.097, 0.240, 0.580, 1.40 mg/L in the geometric ratio: 2.4. The adjustable highest concentration of test solution was 1.40 mg/l. Test vessel was of closed, covered water surface with teflon sheet. Test solution volume was taken as 80 mL/vessel. Total amount of test solution was renewed every day. Number of organisms per vessel was taken as 10 per concentration, one test organism per vessel was taken for study. The number of vessels per concentration (replicates) was taken as ten. Photoperiod was given for 16 hours light with 8 hours darkness. Light intensity provided was less than 800 lux. Test conditions includes the temperature of 20 ±1 °C. Analytical monitoring was done by HPLC. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days NOEC, LOEC and EC50 value of test chemical was determined to be 0.519 mg/l, 1.18 mg/l and > 1.18 mg/L respectively. On the basis of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.

 

The second study of long-term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. the duration of study was 21 days. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days EC50 and NOEC value of test chemical was determined to be 1.2 mg/l and 0.71 mg/l respectively. On the basis of the value of NOEC the test chemical was classified as Chronic category 2 as per CLP criteria.  

 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively. Based on the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.