1,3-Propanediol, 2-[2-(2-amino-6-chloro-9H-purin-9-yl)ethyl]-, 1,3-diacetate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): In-house culture
- Age of inoculum (at test initiation): 3 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22.0 - 22.7 °C

pH:

7.8 - 8.1

Nominal and measured concentrations:

nominal: 1.0, 10 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL, all-glass vessels, containing 50 mL test medium
- Renewal rate of test solution (frequency/flow rate): No renewal, static test
- Initial cells density: 10,000 cells/mL
- Control end cells density: 1,560,000 cells/mL
- No. of vessels per concentration (replicates): 6 replicates for the highest test concentration, 3 replicates for both lower test concentrations, 1 replicate for sampling purposes, 1 - 2 replicates without algae.
- No. of vessels per control (replicates): 6 replicates, 1 replicate for sampling purposes, 1 - 2 replicates without algae.

GROWTH MEDIUM
- Standard medium used: yes, M2 medium according to OECD 201


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-RO water
- Intervals of water quality measurement: pH-value: at the beginning and at the end of the test; temperature: continuously in a temperature control vessel.

OTHER TEST CONDITIONS
- Photoperiod: Continous illumination using TLD-lamps of the type "Cool-white" of 30 Watt
- Light intensity and quality: 95 to 104 µE.m˄-2.s˄-1.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: At the beginning of the test, cells were counted using a microscope and a counting chamber. Thereafter cell densities were determined by spectrophotometric measurement of samples at 720 nm.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50: 0.97 mg/L after 72 h for growth (95% C. I.: 0.62 - 1.5 mg/L)

Reported statistics and error estimates:

Chi-Square Test for Normality, Levene's Test for Homogeneity of Variance, Bonferroni t-Test.