Acetoacetamide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: screening tests

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test wa performed acc to DIN 38412 part 25, similar to OECD 302 B; no GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 25

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acetoacetamide

EC Number:

227-774-4

EC Name:

Acetoacetamide

Cas Number:

5977-14-0

Molecular formula:

C4H7NO2

IUPAC Name:

acetoacetamide

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, non-adapted

Details on inoculum:

Mixture of activated sludge from the sewage treatment plant of the Hoechst AG at Frankfurt am Main (Höchst) with activated sludge fom the sewage treatmant plant at Hoechst AG site Kelsterbach in the ratio 30 : 70

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

357.9 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Stock solution: 5 g 30% acetoacetamide solution were mixed with 1800 ml de-ionized water, the pH adjusted from 6.9 to 7.1. This batch was stirred over a period of 24 hours at 22°C. The resulting clear solution was filled up with de-ionized water to a final volume of 2000 ml and used as stock solution.
Inoculum: Washing the sediment from the activated sludge two times by centrifuging and resuspending in mineral medium. 12 g/L (=1240 mg dry mass/L) was used for the batch.
241 ml/L stock solution was aded. Concentration: 357.9 mg/L

Reference substance

Reference substance 1

Reference substance:

diethylene glycol

Remarks:

concentration: 271.6 mg DOC/L

Reference substance 2

Reference substance:

other: blank control: reaction medium with inoculum but without test or reference substance

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

32

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

70

Sampling time:

9 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

68

Sampling time:

19 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

64

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Diethyleneglycol:
1 d 5%
9 d 82%
16 d 93% DOC removal

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

Acetoacetamide (30% solution) is inherently biodegradable under the conditions of the zahn-Wellens Test.

Executive summary:

A 30% aequous solution of acetoacetamide was tested for inherent biodegradation in the Zahn-Wellens-test by measuring the DOC disappearance. During the first 7 days of the test the DOC exhaustion achieved > 70% of the carbon content of the added test substance, but the following day the rate of degradation staye at the plateau in the range 70 - 72%. The results were confirmed by additionally conducted COD control analyzes. COD at the start was 510 mg/L and 160 mg/L after 28 days, corresponding to an elimination of about 69%.