Allyl cyanoacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976-09-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable for reliability but not in detail documented. Study report meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

No further information available.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Allyl cyanoacetate

EC Number:

236-423-4

EC Name:

Allyl cyanoacetate

Cas Number:

13361-32-5

Molecular formula:

C6H7NO2

IUPAC Name:

prop-2-en-1-yl 2-cyanoacetate

Test material form:

other: liquid

Details on test material:

The test material was mixed with propylene glycol.

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

propylene glycol

Doses:

100 mg/kg
125 mg/kg
160 mg/kg
200 mg/kg
250 mg/kg
320 mg/kg
500 mg/kg
1000 mg/kg
2000 mg/kg
4000 mg/kg

No. of animals per sex per dose:

5 male and 5 female were used for each dose level.

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

160 mg/kg bw

Based on:

test mat.

95% CL:

130 - 200

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

160 mg/kg bw

Based on:

test mat.

95% CL:

120 - 220

Any other information on results incl. tables

Unkempt coats were evident in the animals dosed at 100 mg/kg. The animals dosed at 125 mg/kg and 160 mg/kg were languid with unkempt coats. Lacrimation accompanied unkempt coats and lethargy at levels of 200 mg/kg, 250 mg/kg and 500 mg/kg. Unsteady locomotion, lethargy, lacrimation and unkempt coats were noted in all animals dosed at levels of 1000 mg/kg and above.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LD50 value of Allyl cyanoacetate is 160 mg/kg, therefore the test item will be classified as acute toxic; category 3; oral, H301.

Executive summary:

The study was performed 1976, before GLP- and OECD-testing guidelines were available and in force.

In this assay toxicity was recorded . The LD50 of substance Allyl cyanoacetate is 160 mg/kg and therefore will be classified as acute toxic; category 3 according to GHS/CLP classification criteria.