Toluene-2-sulphonamide

Endpoint summary

Additional information

The substance has no structural alerts for mutagenicity, and a large database of performed studies indicate that o-TSA does not have genotoxic properties.

 

The table below lists all available data, including from literature.

Type of test	Test system	Doses	Result	Reference
Bacterial test
Gene mutation assay	S. typhimurium strains TA98, TA100, TA1535, TA1537, TA1538; Saccharomyces cerevisiae D4	Up to 1000 ug/plate	Negative (+/- S9)	Proprietary, Litton Bionetics, 20838, 1978
Ames test (reverse mutation)	S. typhimurium (TA98, TA100, TA1535, TA1537) E. coli (WP2uvr A)	Up to 5000 ug/plate	Negative (+/- S9)	MHW, Japan: 1999
Ames test (reverse mutation)	S. typhimurium (TA1535, TA100, TA98, TA1537)	Up to 1000 ug/plate	Negative (+/- S9)	Stolts et al.: 1977
Ames test (reverse mutation)	S. typhimurium (TA1530, TA1535, TA1537, TA1538, TA100, TA98)	Up to 4 x 10-2 mol/plate	Negative (+/- S9)	Poncelet et al.: 1979
Ames test (reverse mutation)	S. typhimurium (TA1535, TA100, TA1538, TA98, TA1537)	Up to 18000 ug/plate	Weakly positive: TA98 (+ S9) Negative: TA98 (-S9) and other strains (+/- S9)	Eckhardt et al.: 1980
Ames test (reverse mutation)	S. typhimurium (TA98, TA 100, TA 1535, TA1537)	Up to 18000 ug/plate	Negative (+S9)	Herbold: 1981
Ames test (reverse mutation)	S. typhimurium (TA1535, TA1538, TA98, TA100)	Up to 2500 ug/plate	Negative (+S9)	Ashby et al.: 1978
Non-bacterialin vitrotest
forward mutations (TK locus)	mouse lymphoma L5178Y cells	Up to 1712 μg/mL	Negative (+/- S9)	Proprietary, TNO Triskelion, V20203/05, 2013
Chromosomal aberration test	CHL cells	Up to 3000 ug/mL	Negative (+/- S9)	MHW, Japan:1999
Ouabain-resistant mutation assay	Human RSa cells	Up to 1800 ug/mL	Negative (-S9)	Suzuki & Suzuki: 1988
Chromosomal aberration test	CHO-K1 cells	Up to 400 ug/mL	Negative (-S9)	Masubuchi et al.: 1978
In vivotest
Mammalian spot test	Mouse	1000 mg/kg bw (oral)	Inconclusive	Fahrig: 1982
Micronucleus assay	Mouse	1026 mg/kg bw (by gavage)	Negative	Eckhardt et al.: 1980
Micronucleus assay	Mouse	Up to 1026 mg/kg bw (i.p.)	Negative	Eckhardt et al.: 1980

 

References:

·   Proprietary: Litton Bionetics (Jagannath, D. R. & Brusick, D.), 20838, 1978.

·   Proprietary: TNO Triskelion, V20203/05, 2013

·   Ashby, J. et al. (1978) Fd. Cosmet. Toxicol., 16, 95-103.

·   Eckhardt, K. et al. (1980) Toxicology Letters, 7, 51-60.

·   Fahrig, R. (1982) Mutat. Res., 103, 43-47.

·   Herbold, B.A. (1981) Mutat. Res., 90, 365-372.

·   Masubuchi, M. et al. (1978) Mutat. Res., 54, 242-243.

·   MHW, Japan (1999) Ministry of Health and Welfare, Toxicity Testing Reports of Environmental Chemicals, 7, 125-160.

·   Poncelet, F. et al. (1979) Fd. Cosmet. Toxcol., 17, 229-231.

·   Stolts, D. R. et al. (1977) J. Environ. Pathol. Toxicol., 1, 139-146

·   Suzuki, H. & Suzuki, N. (1988) Mutat. Res., 209, 13-16.

Justification for selection of genetic toxicity endpoint
No one specific study is selected. All key studies for this endpoint addressing bacterial mutagenicity, mammalian mutagenicity and mammalian clastogenicity were all negative.

Short description of key information:
The substance has no structural alerts for mutagenicity, and a large database of performed studies, including high quality GLP studies according OECD guidelines covering in vitro bacterial mutagenicity, mamalian mutagenicity and clastogenicity, as well as in vivo micronucleus studies, indicate that p-TSA does not have genotoxic properties.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

The substance has no structural alerts for mutagenicity, and available studies covering bacterial mutagenicity, mammalian mutagenicity and mammalian clastogenicity all indicate that o-TSA does not have genotoxic properties.