Perylene-3,4:9,10-tetracarboxydiimide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Principles of method if other than guideline:

according to internal standard method

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Perylene-3,4:9,10-tetracarboxydiimide

EC Number:

201-344-6

EC Name:

Perylene-3,4:9,10-tetracarboxydiimide

Cas Number:

81-33-4

Molecular formula:

C24H10N2O4

IUPAC Name:

7,18-diazaheptacyclo[14.6.2.2²,⁵.0³,¹².0⁴,⁹.0¹³,²³.0²⁰,²⁴]hexacosa-1(23),2,4,9,11,13,15,20(24),21,25-decaene-6,8,17,19-tetrone

Details on test material:

Test materials used in this dossier are all considered to fall under the definition of nano-materials according to the European Commission Recommendation 2011/696/EU as the synthesis and manufacturing of this pigment always yields particulate material with a fine particle size distribution.

Test animals

Species:

mouse

Strain:

other: NMRI-Wiga

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: average weight males: 28.7 g, females: 28 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

The test substance was given as a 46.4 or 50% suspension with a 0.5% aqueous carboxymethyl cellulose solution

Doses:

10,000; 6,810; 4,640 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 9 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: mortality was observed

Mortality:

2/5 males and 5/5 females treated with 10,000 mg/kg, and 1/5 females treated with 6,810 mg/kg died.

Clinical signs:

Dyspnea, apathy, unsteady gait and ruffled fur were reported until day 4 in mice given 10,000 and 6,810 mg/kg. Death occurred on day 2 and 3, bad general health until and including day 4. Mice given 4640 mg/kg showed dyspnea and ruffled fur only on the first day of treatment.

Gross pathology:

Diseased animals: intra-abdominal precipitation of the substance and coloration.
Euthanized animals: intra-abdominal precipitation of the substance and coloration, thickening of the edges of the liver.

Any other information on results incl. tables

In a pretest 2 male mice per dose group were treated with dose levels of 215, 1000 and 4640 mg/kg. No deaths occurred, animals of the high and medium dose showed dyspnea and ruffled fur on the first day of treatment.