Methylidynetri-p-phenylene triisocyanate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

version of 1996

Deviations:

no

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methylidynetri-p-phenylene triisocyanate

EC Number:

219-351-8

EC Name:

Methylidynetri-p-phenylene triisocyanate

Cas Number:

2422-91-5

Molecular formula:

C22H13N3O3

IUPAC Name:

1-[bis(4-isocyanatophenyl)methyl]-4-isocyanatobenzene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

SPF-bred Wistar rats of the strain HdsCpd:WU
TEST ANIMALS
- Source: Harlan Winkelmann GmbH, Borchen, Germany
- Age at study initiation: 8-9 weeks
- Weight at study initiation: 225-244 g (males) and 158-164 g (females)
- Fasting period before administration of test substance: about 17 h

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Doses:

2000 mg/kg

No. of animals per sex per dose:

3

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

discriminating dose

Effect level:

ca. 540 mg/kg bw

Based on:

act. ingr.

Remarks on result:

other: calculated from the LD50: > 2500 mg/kg (cut off) determined for the trade product (= a solution of 27% Triphenylmethane-4,4',4''-triisocyanate in ethyl acetate (w/w)

Effect levels 2

Sex:

male/female

Dose descriptor:

other: LD50 (cut off value)

Effect level:

> 2 500 mg/kg bw

Based on:

test mat.

Remarks on result:

other: a dose of 2000 mg/kg bw was tolerated without any findings

Mortality:

no deaths

Clinical signs:

other: none

Gross pathology:

no findings

Other findings:

none

Any other information on results incl. tables

Dose (mg/kg bw)	Toxicological results	Onset and duration of signs	Onset of mortality
male
2000	0 / 0 / 3	---	---
female
2000	0 / 0 / 3	---	---

Toxicological results:

number of dead animals / number of animals with signs after treatment / number of animals treated

Applicant's summary and conclusion

Executive summary:

The acute oral toxicity of the Triphenylmethane-4,4',4''-triisocyanate (administered as a 27% solution in ethyl acetate) was evaluated in a GLP-compliant study on male and female Wistar rats according to OECD TG 423 of 1996. The oral dose of 2000 mg solution/kg bw was tolerated without mortalities, clinical signs, effects on weight gain or gross pathological findings. According to OECD TG 423 the LD50 cut-off of the solution can therefore be determined with > 2500 mg solution/kg bw. With regard to the content of Triphenylmethane-4,4',4''-triisocyanatet this is equivalent to approx. 540 mg/kg .