Registration Dossier
Registration Dossier
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EC number: 277-143-2 | CAS number: 72968-50-4 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Citrus aurantium, Rutaceae.
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The 72h-EL50 is read across from Lime oil and is 8.0 mg/L and the EL10 is 5.1 mg/l (Loading rates, WAF study)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8 mg/L
- EC10 or NOEC for freshwater algae:
- 5.1 mg/L
Additional information
For orange oil bitter, no studies on the aquatic toxicity are available for algae. For this endpoint, read-across is conducted to the citrus group. Key WAF studies are available for orange oil, mandarin oil and lime oil, see summary in the table.
Test substance (Read across) |
Endpoint (growth) |
Remarks |
Reference |
Lime Oil Distilled |
72h-EL50 = 8 mg/l (WAF) 72h-EL10 = 5.1 mg/l (WAF) |
OECD 201, GLP, Rel. 2, KEY |
Harlan 2011 |
Mandarin Oil |
72h-EL50 = 9.7 mg/l (WAF) 72h-EL10 = 7.8 mg/l (WAF) |
OECD 201, GLP, Rel. 2, |
Harlan 2011 |
Lime Oil Cold Pressed |
72h-EL50 = 11 mg/l (WAF) 72h-EL10 = 2.2 mg/l (WAF) |
OECD 201, GLP, Rel. 2, |
Harlan 2011 |
Orange Oil |
72h-EL50 = 150 mg/l 72h-NOELr = 50 mg/l |
OECD 201, GLP, Rel. 2, |
Lebertz 2007 |
For the data gap of bitter orange oil these studies are considered suitable for read-across and therefore given Reliability 2. The WAF toxicity values (ErL50) for Mandarin oil and both Lime oils are very similar, while for orange oil the WAF result showed an ErL50 10 times higher (less toxic).
The study with Lime oil distilled constitutes the lowest toxicity value (lowest WAF value, highest toxicity) for this category and is selected as key study. The values for Orange oil Mandarin oil and the other quality of Lime oil are used as supporting studies.
Remark:
In the tests of Harlan (2011) and Lebertz (2007) the concentration of limonene and total hydrocarbons, respectively, were measured. As these oils are all complex test substances that contain a range of constituents, the dose rates were prepared as Water Accommodated Fractions (WAF). The analytical measurements were be used to establish the stability of the test solutions over time. They should not be used to express the toxicity of the Citrus Oils on the basis of a single substance or sum parameter.
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