Registration Dossier
Registration Dossier
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EC number: 929-442-0 | CAS number: 1040871-35-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl 3-mercaptopropionate
- EC Number:
- 220-912-4
- EC Name:
- Methyl 3-mercaptopropionate
- Cas Number:
- 2935-90-2
- Molecular formula:
- C4H8O2S
- IUPAC Name:
- methyl 3-sulfanylpropanoate
- Details on test material:
- IUCLID4 Test substance: as prescribed by 1.1 - 1.4
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- S. typhimurium TA 1537
- Species / strain / cell type:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 61.7, 185.2, 555.6, 1666.7, 5000 ug/plate.
- Evaluation criteria:
- Criteria for positive response are minimum two-fold increase
in revertants compared to solvent control over three dose levels with appropriate response in positive and negative
control responses.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Without Activation:
CONC (ug/
plate) TA1535 TA1537 TA1538 TA100 TA98
1667 6.3+/-2.3 5.7+/-1.2 9.7+/-2.1 88.7+/-9.1
38.3+/-11.0
555.6 9.7+/-6.7 9.7+/-3.1 9.3+/-4.9 12.8+/-13 62.7+/-1.6
185.2 12.3+/-0.6 6.7+/-2.3 13.0+/-1.0 15.0+/-25 42.0+/-9.0
61.7 19.3+/-15 5.7+/-2.1 12.3+/-3.8 15.4+/-11 42.3+/-9.5
Positive 1795+/-160 5201+/-78.8 1532+/-159 22421+/-101
22531+/-34
Control
DMSO 16.3+/-2.9 8.7+/-4.0 12.7+/-5.0 11.9+/-15 49.7+/-3.1
With Activation:
CONC (ug/
plate) TA1535 TA1537 TA1538 TA100 TA98
1667 7.0+/-1.7 6.0+/-2.0 21.0+/-2.6 12.3+/-20
46.0+/-9.5
555.6 14.3+/-2.1 13.3+/-1.2 21.7+/-7.5 130.0+/-5.0
41.0+/-8.7
185.2 9.7+/-2.1 12.3+/-1.2 24.3+/-9.9 135.0+/-7.0 35.3+/-13
61.7 17.7+/-2.5 8.0+/-3.0 26.3+/-2.1 145.1+/-4.5
38.7+/-2.5
Positive2831+/-4.5 3281+/-35 2009+/-166 2253+/-154
2196+/-177
Control
DMSO 18.3+/-1.5 8.3+/-2.3 27.0+/-3.0 135.1+/-4.3
23.7+/-8.1
Applicant's summary and conclusion
- Conclusions:
- Exposure to five graded doses of the test material in the presence of metabolic activation and in
the absence of metabolic activation did not increase the reversion to histidine prototrophy S. typhimurium strains TA1535, TA1537, TA1538, TA98 or TA100. The test system met all the criteria established for evaluation of an unknown agent. Therefore, methyl-3-mercaptopropionate is not considered to be mutagenic in this test system.
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