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Environmental fate & pathways

Biodegradation in water: screening tests

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Referenceopen allclose all

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, Basic data given.
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
GLP compliance:
not specified
Inoculum or test system:
mixture of sewage, soil and natural water
Details on inoculum:
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
4 wk
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
test equipment: standard type
Parameter:
% degradation (O2 consumption)
Value:
48
Sampling time:
4 wk
Remarks on result:
other: reported as BOD
Parameter:
% degradation (TOC removal)
Value:
50
Sampling time:
4 wk
Parameter:
% degradation (test mat. analysis)
Value:
86
Sampling time:
4 wk
Remarks on result:
other: method: GC
Details on results:
The test substance partially remained unchanged in the test solution, while the rest changed form to methanol, phosphonic acid, and monomethyl phosphonate.
Interpretation of results:
other: not ready biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP - Guideline study, basic data given
Qualifier:
according to guideline
Guideline:
EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)
Version / remarks:
, Directive 79/831/EEC, Annex V
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/ activated sludge: sewage of a laboratory treatment plant which operates with municiple waste water
- Concentration of sludge: 0.5 ml/900 ml
- no pre-treatment
- sampling: 18.02.1992
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 20-24°C
- Continuous darkness: yes

SAMPLING
- Sampling frequency: samples were taken at the start of the test (0 h) and following on day 7, 14, 21, 27 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Reference substance:
aniline
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
0 h
Parameter:
% degradation (DOC removal)
Value:
44
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
50
Sampling time:
14 d
Parameter:
% degradation (DOC removal)
Value:
50
Sampling time:
21 d
Parameter:
% degradation (DOC removal)
Value:
50
Sampling time:
28 d
Results with reference substance:
aniline: 100 % degradation after 28 days
Interpretation of results:
other: not readily biodegradable

Description of key information

Dimethyl phophonate (DMP,) one of the main components of phosphitester residues, shows some degradation but is not considered as ready biodegradable. As worst case approach, also phosphitester residue is considered as not readiyl biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information