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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: A scientific study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992

Materials and methods

Principles of method if other than guideline:
The test method carried out is based on and similar to that described by Magnusson and Kligman (1970).
GLP compliance:
not specified
Type of study:
guinea pig maximisation test

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzothiazole-2-thiol
EC Number:
205-736-8
EC Name:
Benzothiazole-2-thiol
Cas Number:
149-30-4
Molecular formula:
C7H5NS2
IUPAC Name:
1,3-benzothiazole-2-thiol
Constituent 2
Reference substance name:
Mercaptobenzothiazole
IUPAC Name:
Mercaptobenzothiazole
Details on test material:
Source: Aldrich
Purity: 98%

In vivo test system

Test animals

Species:
guinea pig
Strain:
other: Albino Dunkin-Hartley
Sex:
not specified
Details on test animals and environmental conditions:
Guinea-pigs weighed approximately 350 g at the start of the study.

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Route:
intradermal
Vehicle:
other: acetone-polyethylene glycol 400 (70:30, v/v).
Concentration / amount:
10%
Challengeopen allclose all
Route:
epicutaneous, occlusive
Vehicle:
other: acetone-polyethylene glycol 400 (70:30, v/v).
Concentration / amount:
10%
No. of animals per dose:
No data
Challenge controls:
After 6-8 days, sensitization was boosted by a 48-hr occluded patch placed over the injection site. 12-14 days later, the animals were challenged on one flank by a 24-hr occluded patch at the maximum non-irritant concentration. Challenge sites were scored for erythema (scale 0-3) and oedema 24 and 48 hr after patch removal.
Positive control substance(s):
not specified

Study design: in vivo (LLNA)

Vehicle:
dimethylformamide
Concentration:
10, 25, 50 %
No. of animals per dose:
No data

Results and discussion

In vivo (non-LLNA)

Results
Reading:
1st reading
Hours after challenge:
24
Group:
test chemical
Dose level:
10%
Clinical observations:
80% of test animals judged to be positive at 24 and/or 48 hr
Remarks on result:
other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 10%. Clinical observations: 80% of test animals judged to be positive at 24 and/or 48 hr.

In vivo (LLNA)

Results
Parameter:
other: disintegrations per minute (DPM)
Remarks on result:
other: 4.5, 4.6, 5.5

Any other information on results incl. tables

The Magnusson and Kligman guinea-pig maximization test was carried out based on and similar to that described by Magnusson and Kligman. The concentration was a 10% of the test material. The 80% of the test animals judged to be positive (skin sensitisation).

Applicant's summary and conclusion

Interpretation of results:
sensitising
Remarks:
Migrated information
Conclusions:
According to Guinea-pig maximization test the test substance is sensitizer.
Executive summary:

The Magnusson and Kligman guinea-pig maximization test was carried out based on and similar to that described by Magnusson and Kligman. The concentration was a 10% of the test material. The 80% of the test animals judged to be positive (skin sensitisation).