[No public or meaningful name is available]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 03 March 2020 and 31 March 2020.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study is considered to be a reliability 1 as it has been conducted according to OECD Test Guideline 301F using a Manometric Respirometry method and in compliance with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of 2(R/S)‐1‐{[(1R,2S,5R,8S)‐4,4,8‐trimethyltricyclo[6.3.1.0~2,5~]dodecan‐1‐yl]oxy}pentan‐2‐o and 2(R/S)‐1‐{[(1S,2S,5R,8R)‐1,4,4‐trimethyltricyclo[6.3.1.0~2,5~]dodecan‐8‐yl]oxy}pentan‐2‐ol

Molecular formula:

C20H36O2

IUPAC Name:

Reaction mass of 2(R/S)‐1‐{[(1R,2S,5R,8S)‐4,4,8‐trimethyltricyclo[6.3.1.0~2,5~]dodecan‐1‐yl]oxy}pentan‐2‐o and 2(R/S)‐1‐{[(1S,2S,5R,8R)‐1,4,4‐trimethyltricyclo[6.3.1.0~2,5~]dodecan‐8‐yl]oxy}pentan‐2‐ol

Test material form:

liquid: viscous

Details on test material:

Chemical name: 1‐[(4,4,8‐trimethyltricyclo[6.3.1.02,5]dodecan‐1‐yl)oxy]pentan‐2‐ol and 1‐[(1,4,4‐trimethyltricyclo[6.3.1.02,5]dodecan‐8‐yl)oxy]pentan‐2‐ol and isomers

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge was obtained as the test inoculum from a sewage plant treating predominantly domestic sewage (Nanjing Tiebei Sewage Treatment Plant). On return to the laboratory, coarse particles were removed by filtration through a fine sieve and the sludge aerobic will be kept thereafter for 5-7 days at the test temperature.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

> 50 - < 100 other: mg/L

Based on:

COD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The vessels will be allowed to reach the desired temperature and appropriate vessels will be inoculated with prepared activated sludge to give a concentration of suspended solids at 30 mg/L.
Addition of Test Substance – appropriate amount of test substance will be added into test bottles of “test” (bottle 1&2) and “Inhibition control” (bottle 6).
Addition of Reference Substance – 10 mL of the reference control stock solution of Sodium benzoate (1000 mg/L) will be added into test bottles of “procedure control” (bottle 5) and “Inhibition control” (bottle 6) to give a final concentration of 40 mg/L, respectively.
Addition of Inoculums – 1.875 mL of Inoculums suspended solids will be added into “test” (bottle 1&2), “inoculum control” (bottle 3&4), “procedure control” (bottle 5) and “Inhibition control” (bottle 6) to give a concentration of 30 mg/L.
Basal Salts Medium (BSM) will be added to every test bottle to get a final volume of 250 mL.
The equipment will be assembled, the stirrer started, the equipment checked that it is air-tight, and the measurement of oxygen uptake will be started (Time zero). Carbon dioxide produced metabolically by the bacteria is chemically bound by the sodium hydroxide solution contained in the seal cup in the bottle. Usually no further attention is required other than taking the necessary readings and making daily checks to see that the correct temperature and adequate stirring are maintained. According to the readings of automatic respirometer, a continuous record of oxygen uptake is obtained so that the 10-d window is easily recognized.
The oxygen uptake will be calculated from the readings taken at least daily, using the methods given by the manufacturer of the equipment.
At the beginning and the end of incubation, normally 28 days, the pH of the contents of the flasks will be measured

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 95

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Under the valid conditions, biodegradation of the FRET 18-0091 was 95.0% at the end of the 28-day study.

Executive summary:

Under the valid conditions, biodegradation of the FRET 18-0091 was 95.0% at the end of the 28-day study.