[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From February 20 to March 26, 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch number: CN0191205
Purity: 98.3%

Analytical monitoring:

yes

Details on sampling:

Samples were taken for analysis from the freshly prepared solutions at 0 hour and the old solutions at 48 hours.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0503 g test item was weighed and dissolved in 500 mL of test water by magnetic stirring for 24 hours. After magnetic stirring, undissolved test item was observed in the solution, the solution was filtered through a 0.45 μm filter membrane and the filtrated solution was used to dilute other test solutions. Pipette 25.0, 40.0, 67.5, 107.5 and 175.0 mL of the filtrated solution and make up to 250 mL with test water. The solutions were mixed thoroughly, resulting in test solutions at 10%, 16%, 27%, 43% and 70% of the saturated solution prepared at 100 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation (mean and range, SD): less than 24 hours old and not the first brood progeny.
- Source: Daphnia of stock culture was originally obtained from Applied Chemistry Department Pesticide Environmental Evaluation Laboratory of China Agricultural University.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

140 to 250 mg/L (as CaCO3)

Test temperature:

18~22oC

pH:

6.0 to 8.5

Dissolved oxygen:

not less than 3 mg/L during the test period

Nominal and measured concentrations:

nominal: 10%, 16%, 27%, 43% and 70% of the saturated solution prepared at 100 mg/L;
first definitive test: 2.38, 3.95, 6.92, 11.5 and 19.3 mg/L
second definitive test: 4.74, 7.31, 11.2, 17.1 and 25.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 200 mL glass beakers
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Volume of solution: 50 mL per vessel
- Aeration: Before usage, the water was aerated for more than 24 hours to attain dissolved oxygen saturation.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5 daphnia
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water was tap water after being treated with Aquapro Pure Water System
- Total organic carbon: < 2 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hr light: 8 hr dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Biological observation: Observation intervals: 24 and 48 hours after exposure

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 1%, 5%, 10% and 50% of the saturated solution prepared at 100 mg/L
- Results used to determine the conditions for the definitive study: During the period of 48 hours, no immobilization or any toxic signs were observed in the control group and the test groups of 1%, 5% and 10% of 100 mg/L. 100% immobilization occurred in the test groups of 50% and 100% of 100 mg/L after 48 hours.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

23.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

During the 48-hour exposure period of the first definitive test, no immobilization and abnormal behavior or appearance was observed in the control group and all test groups.
During the 48-hour exposure period of the second definitive test, no immobilization and abnormal behavior or appearance was observed in the control group and the test groups of 20%, 30%, 45%, 67% of the saturated solution prepared at 100 mg/L, the daphnids exposed to the test group of 100% of the saturated solution prepared at 100 mg/L showed abnormal behavior such as hypoactivity and immobilization.

Results with reference substance (positive control):

EC50-24 h of potassium dichromate to Daphnia magna was 1.44 mg/L, and the 95% confidence limits was 1.23 ~ 1.69 mg/L. The results met the recommended criterion in OECD Guideline No. 202: EC50-24 h of potassium dichromate is within the range of 0.6 mg/L to 2.1 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 of the test item PF-06452787 (BOC Ketone) to Daphnia magna was determined to be 23.8 mg/L.

Executive summary:

A Daphnia acute immobilisation test of PF-06452787 (BOC Ketone) was conducted with Daphnia magna over a 48-hour exposure period.

The 48-hour EC50 of the test item PF-06452787 (BOC Ketone) to Daphnia magna was determined to be 23.8 mg/L.

Description of key information

A Daphnia acute immobilisation test of PF-06452787 (BOC Ketone) was conducted with Daphnia magna over a 48-hour exposure period.

The 48-hour EC50 of the test item PF-06452787 (BOC Ketone) to Daphnia magna was determined to be 23.8 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

23.8 mg/L