Ethanol, 2-amino-, reaction products with ammonia, by-products from, distn. Residues

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20-03-1990 to 25-03-1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study is complete, performed under GLP and valid. however no analysis was performed and thus the usability for riskassessment purposes is limited when assessing the bioavailable fraction.

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

For the definitive test a stock solution of 1. 0 g/litre was
prepared by dissolving the test substance in deionized water.
This stock solution was titrated to a neutral pH. The selected
test concentrations were prepared by dilution of this stock
solution in the inoculated test medium. Based on the results of
preliminary experiments the definitive test were selected.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

The test was carried out with the unicellular green algae;
Selenastrum capricornutum ATCC 22662. This strain was maintained
on mineral salts agar slants in the light at room temperature.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Nominal and measured concentrations:

nominal: 2.5, 5.0, 10.0, 20.0 and 40.0 mg/litre

Details on test conditions:

- The KH2PO4 and NaC03 concentrations in the test medium were
increased to 160 and 100 mg/litre, respectively, to maintain a
constant pH.
- Preliminary experiments to determine the concentration range
are performed but not reported according to the guidelines.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

< 2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The results of the extinction measurements are presented in
in the report. Both the
ErC50 and EbC50 (0-72 h) of triethylenetetramine are 20 mg/litre.
The.NOEC determined of triethylenetetramime is < 2.5 mg/litre.

Results with reference substance (positive control):

within validity criteria

Reported statistics and error estimates:

according to guideline

EC10 was determined with Toxcalc to be 1.34 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of TETA was tested to S. capricornutum, both EC50 values were determined to be 20 mg/L and the NOEC was <2.5 mg/L. the study meets all criteria and thus is valid. However no analysis was performed and thus the use in riskassessments is restriced.

Executive summary:

The toxicity of triethylenetetramine to exponentially growing S. capricornutum has been assessed over an exposure period of 72 hours, according to the OECD 201 guideline. In the definitive test the algae were exposed to the following nominal concentrations of 2.5, 5.0, 10.0, 20.0 and 40.0 mg/litre. Both the ErC50 (0 -72 h) and EbC50 of triethylenetetramine for S. capricornutum are 20 mg/litre. The NOEC determined of triethylenetetramine is < 2.5 mg/L. the test is valid and meets all crietria. No analysis was performed.

Description of key information

Based on read across from data generated on the major constituent, triethylenetetramine,  the toxicity to algae is expected to be in the range  10 - 100 mg/L.

Additional information

No studies on the actual substance are avilable. However results from tests performed on triethylenetetramine, the major constituent is available. This result indicates that the toxicity to algae is in the range 10 - 100 mg/L