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EC number: 700-235-5 | CAS number: 174899-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study Plan dated: 19.Sep.2018; Experimental starting Date: 19.Mar.2019; Experimental Completion Date: 26.Mar.2019
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 23. March 2006; Annex 5 corrected: 28. July 2011
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 07. December 2015
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guidance document no. 23, GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES
- Version / remarks:
- 06. Jul. 2018
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt; Kaiser-Friedrich-Straße 7, 55116 Mainz
Test material
- Reference substance name:
- 1-ethyl-3-methyl-1H-imidazol-3-ium; trifluoro[(trifluoromethanesulfonylazanidyl)sulfonyl]methane
- EC Number:
- 700-235-5
- Cas Number:
- 174899-82-2
- Molecular formula:
- C8H11F6N3O4S2
- IUPAC Name:
- 1-ethyl-3-methyl-1H-imidazol-3-ium; trifluoro[(trifluoromethanesulfonylazanidyl)sulfonyl]methane
- Test material form:
- liquid
- Details on test material:
- clear liquid, colorless
Constituent 1
- Specific details on test material used for the study:
- Source and lot/batch No.of test material: proionic GmbH; Lot.-No.: 25PI208_12
- Expiration date of the lot/batch: 01.May 2019
- Purity test date: EMIM+: 99.84 wt% HPLC; NTF2-: 99.95 wt% IC
- Storage condition of test material: room temperature (20 +/- 5 °C), keep away from humidity
T
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- LC-MS-MS
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): MBM Sciencebridge GmbH
- Method of cultivation: stock culture on solid agar
Study design
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.8 - 24.3 °C
- pH:
- Nominal Concentration in mg/L 0 h 72 h
Blank control 7.9 7.8
0.1* 7.9 7.8
0.32* 7.9 7.8
1 7.9 7.8
3.2 7.9 7.8
10 7.9 7.8 - Details on test conditions:
- Date: 19. – 22. March 2019
Treatments tested: 0.1 / 0.32 / 1 / 3.2 / 10 mg/L nominal concentration
The concentrations to be tested are based on non GLP pre-tests
Number of replicates: 6 replicates for the blank control
3 replicates for each treatment
Vessels: glass flasks total volume 65 mL
Duration: 72 hours
Lighting: 5000 lux
Temperature: 22.8 – 24.3°C (see chapter 13 Deviations)
Replicates (Blank control): 45 ±1 mL algal medium and algae
Replicates (Treatments): 45 ±1 mL test solution and algae - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7; CAS No. 7778-50-9)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13.17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- The results of the last study with the positive control K2Cr2O7 are presented in the follow-ing table. The study was performed under GLP conditions in June 2018.
Table Biological Results of K2Cr2O7
Parameter Value 95% confidence interval
72h ErC50 0.65 mg/L 0.60 mg/L – 0.71 mg/L
72h EyC50 0.28 mg/L 0.21 mg/L – 0.38 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 0.1 to 10 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield[1] were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 10 mg/L
As no inhibition occurred in the three lowest treatments, only the three highest concentrations (1 – 10 mg/L) would be used for evaluation.
At the start and at the end of the test, the content of the test item in the test solutions was determined using LC-MS-MS.
The measured concentrations of the three highest treatments lay between 85 % and 92 % of the nominal concentrations at the beginning of the test and between 92 % and 100 % of the nominal concentrations at the end of the test. Therefore, the biological results were based on the nominal concentrations (cf. Guidance Doc. No. 23 §176).
The 72h-EC50values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.65 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test item1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imidewere determined:
Table: Results of the test item
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
1.00 mg/L
3.20 mg/L
4.70 mg/L
13.17 mg/L
Yield
1.00 mg/L
3.20 mg/L
2.50 mg/L
5.19 mg/L
[1]Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 8.2 Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. Algal biomass is defined as the dry weight per volume, e.g. mg algae/L test solution. However, dry weight is difficult to measure and therefore surrogate parameters are used. Of these surrogates, cell counts are most often used.
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