methyl (2E)-{2-[(2,5-dimethylphenoxy)methyl]phenyl}(methoxyimino)ethanoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Valid without restriction

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Name of the test substance: 505-Oximether
Purity: 84.7 % w/w

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentration was prepared by diluting an acetonic stock solution with dilution water.
The stock solution was prepared by weighing 0.0506 g test substance and diluting it in 100
mL acetone. This stock solution was further diluted with acetone to recieve stock solutions for
each test concentration (see scheme below). The required amount of the acetonic stock
solution was given into the test vessel. After evaporating of the acetone the test vesseis wer~
filled with test water. The test substance was dissolved in the test water using an ultra turraxl.*
lt was tried to maximize the recovery rates by a prolonged stirring period of 5 days before the
fish were inserted into the test solutions to reach the maximum solubility, since an increase in
the test concentrations was observed in another aquatic study over 3 days. However, in this
test no marked increase was observed during the 5 days stirring period and a decrease of
the concentrations was observed at the end of the exposure period.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Test species: Zebrafish (Danio rerio)
- Animal suppier: Zierfisch-Center Klöckner, Ludwigshafen, Germany
- Body weight (wet weight): 0.30 g (0.24 - 0.42 g)
- Body length (from the top of the snout 3.3 cm (3.1 - 3.6 cm) to the end of the caudal fin):
- Age of the animals: Approx. 4 months

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Details on test conditions:

- Dilution water source: Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter.
- Total hardness: about 1 mmol/l
Acid capacity: about 2.5 mmol/l
pH: 7.5 - 8.5
Ca content : about 40 mg/l
Mg content : about 5 mg/l
- Exposure vessel type: Test vessels: All glass aquaria (30 x 22 x 24 cm)
- Volume of water: 10 l
- Aeration: Continuous aeration with oil-free air, slight
- Photoperiod: 16 : 8 hours day-night regime
- One test vessel per treatment
- Number of animals per test concentration and control: 10
- pH during the test: 7.8 - 8.2
- Oxygen content during the test: 7.9 - 8.8 mg/l
- Water temperature: 22 - 23 °C;
- Duration of adaption: about 2 weeks Mortality during the adaption period: 0 %
- Withdrawal of food: 1 day before and during exposure.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

0.066 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.041 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.026 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
Nominal concentrations (mg/l): 0.022, 0.05, 0.1, 0.22 and 0.5

An analytical dose-verification of the test item was carried out at day 1.

Nom. Conc. (mg/l) Mean Anal. Conc. (mg/l) % of Nom. Conc.
0 n.d. -
0.022 < loq -
0.05 0.0103 20.6
0.1 0.0188 18.8
0.22 0.0394 17.9
0.5 0.1047 20.9
(n.d. = not detectable, loq = limit of quantification)

LC 50 (48h): 0.041 mg/l
LC 50 (72h): 0.041 mg/l
LC 50 (96h): 0.041 mg/l


Biological observations:
St = stottering, D = swimming at the bottom, C = swimming at the surface, A = apathy

Table showing cumulative mortality:
Cumulative mortality

Nom. conc. (mg/l) 1h 4h 24h 48h 72h 96h
Control 0 0 0 0 0 0
0.022 0 0 0 0 0 0
0.05 0 0 0 0 0 0
0.1 0 0 0 0 0 0
0.22 0 0 0 0 0 0
0.5 0 3 8 10 10 10


Behavior of the test item during the test: No remarkable observations

Applicant's summary and conclusion

Validity criteria fulfilled:

yes