Tri(tridecyl) benzene-1,2,4-tricarboxylate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Manometric respirometry test OECD 301F

Principles of method if other than guideline:

- Principle of test: Manometric Respirometry test OECD 301F
- Short description of test conditions: The biodegradability was assessed by measuring the consumption of oxygen by a microbial population (activated sludge) exposed to the test item under controlled conditions.
- Parameters analysed / observed: Comsumpton of oxygen

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Tri(tridecyl) benzene-1,2,4-tricarboxylate

EC Number:

302-446-4

EC Name:

Tri(tridecyl) benzene-1,2,4-tricarboxylate

Cas Number:

94109-09-8

Molecular formula:

C48H84O6

IUPAC Name:

tri(tridecyl) benzene-1,2,4-tricarboxylate

Test material form:

liquid

Details on test material:

- Moisture content: <= 0.1%

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: lot P-2960F-10
- Expiration date of the lot/batch: November 2013
- Purity test date: > 99%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: insoluble in water. The test and reference items were added suing silica gel as a solid matrix.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: insoluble in water. The test and reference items were added suing silica gel as a solid matrix.
- Final dilution of a dissolved solid, stock liquid or gel: the Concentration corresponded to a 20:1 ratio of silica gel to test item.


FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Name and location of sewage treatment plant where inoculum was collected: Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Pretreatment: Sludge was centrifuged, supernatant discarded, and sludge pellet re-suspended in mineral medium twice; sludge was brought up to the original volume; aerated in teh dark at test temperature until use
- Initial biomass concentration:0.02g/L

Study design

Test type:

other: aerobic

Water media type:

other: sludge

Total exposure duration:

28 d

Test conditions

Test temperature:

22.1 +- 0.8°C

Details on test conditions:

TEST SYSTEM
- Test vessel: 2 L falt-bottomed glass bottles
- Type (delete if not applicable): open / closed closed
- Material, size, headspace, fill volume: test volume 1500 mL
- Aeration: no
- No. of vessels per concentration (replicates): 2 (inoculum blank and test item)
- No. of vessels per control (replicates): 1 (Reference Item and Toxicity control)
- No. of vessels per vehicle control (replicates): 1
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 26.5 mg/L
- Nutrients provided for bacteria: see report

OTHER TEST CONDITIONS
- Photoperiod: in the dark or dim light (except during short dailey readings)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : consumption of O2

Reference substance (positive control):

yes

Remarks:

Canola oil

Results and discussion

Effect concentrations

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

ca. 37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

other: toxicity

Details on results:

- Blank controls oxygen uptake rate: Canola Oil
- Coefficient of variation of oxygen uptake rate in control replicates:

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The percentage degradation of the toxicity control (containing both test and reference items) reached 73%. Given that the value seen in the toxicity control is similar to that of the reference item alone (67%), it is unlikely that the test item had a significant effect on the microorganisms ability to utilise the reference item for metabolism.
NOEC = 37 m/g/L