Fatty acids, coco derivs., reaction products with glycine, potassium salts

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21.06.2012-19.07.2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Auf dem Aberg 1, D-57392 Schmallenberg, Germany

Test material

Test material information

Constituent 1

Reference substance name:

dipotassium 2-dodecanamidoacetate 2-tetradecanamidoacetate

EC Number:

620-582-5

Cas Number:

301341-58-2

Molecular formula:

R-CO-NH-CH2-COOK (Note: "R-CO-" is a fatty acid residue)

IUPAC Name:

dipotassium 2-dodecanamidoacetate 2-tetradecanamidoacetate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Acylglycinate GCK-11-T
- Physical state: White to pale yellow powder, a slightly characteristic odor
- Lot/batch No.: 10825
- Expiration date of the lot/batch: August 25, 2014
- Storage condition of test material: Dark and cool place, protected from light
- ThoD: 2.060 mg O2/mg

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Ajinomoto Co., Inc., 15-1 Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315 Japan/Batch No.: 110825
- Expiration date of the lot/batch: 25.08.2014
- Purity test date: 14.03.2012

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: dark and cool place, protected from light

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no

FORM AS APPLIED IN THE TEST (if different from that of starting material): solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Fresh samples of activated sludge were withdrawn from the sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, Germany. The sewage treatment plant was mainly fed with municipal wastewater
- Method of cultivation: samples were not washed with mineral medium after the arrival at the laboratory but kept aerobic until use
- Initial cell/biomass concentration: 29.6 mg dry mass/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

100 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

206 mg/L

Based on:

ThOD/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 °C ± 1°C
- pH: test start: 7.4± 0.2, test end: 7.2-7.8 (in all test vessels)
- pH adjusted: yes
- Aeration of dilution water: not reported
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: respirometer
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration during the test
- Measuring equipment: Sapromat

SAMPLING
- Sampling frequency: oxygen demand measured continuously via Sapromat respirometer

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes (sterilized with HgCl2)
- Toxicity control: yes
- Other: Procedural control using sodium benzoate was performed

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/L

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

85.9

St. dev.:

1

Sampling time:

14 d

Remarks on result:

other: mean of 2 determinations

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

93.7

St. dev.:

2.7

Sampling time:

28 d

Remarks on result:

other: mean of 2 determinations

BOD5 / COD results

Results with reference substance:

The reference item sodium benzoate was degraded to 88.6 % within the first 14 days thus fulfilling the guideline requirements.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Acylglycinate GCK-11-T proved to be readily biodegradable. Furthermore, the test material fulfils the 10d-window (93.7% biodegradation after 28 d).

Executive summary:

The ready biodegradation of the test material was investigated in a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) and EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test) over a period of 28 days and using non-adapted activated sludge as inoculum. The biodegradation rate was determined by measurement of oxygen consumption. Inoculum blank, procedural/functional control with reference substance sodium benzoate and toxicity control using 100 mg/L test item and 100 mg/L reference compound were performed.

 

This study is regarded as reliable without restriction and satisfies the guideline requirements for ready biodegradation.

The test item proved to be readily biodegradable and fulfilling the 10-d-window criterion (93.7% biodegradation after 28 d). The functional control reached the pass level > 60% after 14 d. In the toxicity control containing both test and reference item 87.9% biodegradation based on ThOD occurred within 14 d thus indicating that the test item was not inhibitory to the inoculum at the concentration tested.