Cuprate(4-), [C-(aminosulfonyl)-C-[[[2-[[4-chloro-6-[(2,5-disulfophenyl)amino]-1,3,5-triazin-2-yl]amino]ethyl]amino]sulfonyl]-29H,31H-phthalocyanine-C,C-disulfonato(6-)-N29,N30,N31,N32]-, sodium salts

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 May - 24 June 1993.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

OECD Guidelines for Testing of Chemicals (1981) No. 301D "Ready Biodegradability ; Closed Bottle Test"

Deviations:

not specified

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

Method C.6 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC) .

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

No further details specified in the study report.

Oxygen conditions:

anaerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Type: Activated sludge bacteria.
Source: The aeration stage of the Severn Trent Water Plc sewage treatment plant at Belper, Derbyshire, treating predominantly domestic sewage.
Date of collection: 27 May 1993.
Preparation: The sample was homogenized for 5 minutes and the suspended solids allowed to settle for approximately 30 minutes. The supernatant was then centrifuged to remove any fine solids prior to use.

Duration of test (contact time):

28 d

Initial conc.:

3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Vessels: 250-300 ml BOD bottles (darkened glass) with ground glass stoppers.

Test concentrations:
(a) Non - inoculated culture medium .
(b) Inoculated culture medium.
(c) 3 mg/l test material
(d) 3 mg/l sodium benzoate.
Test media b, c and d were inoculated with activated sludge filtrate at the rate of 1 drop of inoculum per litre.

Procedure: BOD bottles were filled by siphon and firmly stoppered to exclude all air bubbles. Sufficient bottles were prepared to allow a single oxygen determination per bottle to be made at 0, 5, 15 and 28 days for each test medium (duplicate bottles at each sampling time).
Bottles were incubated in a water bath at 21 °C for 0, 5, 15 or 28 days as appropriate.

Observations: Dissolved oxygen concentrations for each test medium were determined, in duplicate, at 0, 5, 15 and 28 days by means of a Yellow Springs BOD Probe (Model 54).

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

JPR Blue 100 attained 0% degradation after 28 days and so, therefore, cannot be considered as ready biodegradable under the strict terms and conditions of OECD Guideline No . 3010.

Results with reference substance:

Sodium benzoate attained 84% degradation after 28 days thereby confirming the suitability of the test method and culture conditions.

Dissolved Oxygen Measurements (mg O₂/l)

Test Series		DAY
		0	5	15	28
(a) Culture medium without inoculum	R1 R1 Mean	8.90 8.90 8.900	8.90 8.90 8.900	8.70 8.80 8.750	8.70 8.65 8.675
(b) Culture medium with inoculum	R1 R2 Mean	8.90 8.90 8.900	8.85 8.90 8.875	8.60 8.60 8.600	8.50 8.55 8.525
(c) Test material, JPR Blue 100 (3 mg/l) with inoculum	R1 R2 Mean	8.90 8.90 8.900	8.90 8.80 8.850	8.70 8.70 8.700	8.55 8.60 8.575
(d) Standard material, Sodium benzoate (3 mg/l) with inoculum	R1 R2 Mean	8.90 8.90 8.900	5.45 5.35 5.400	4.90 5.10 5.000	4.20 4.40 4.300

R₁and R₂= Replicates 1 and 2

 

Mean Oxygen Depletion and Percentage Biodegradation Values

Test Series		DAY
		5	15	28
(a) Culture medium without inoculum	} O2depletion (mg O2/l) } % degradation	0.000 -	0.150 -	0.225 -
(b) Culture medium with inoculum	} O2depletion (mg O2/l) } % degradation	0.025 -	0.300 -	0.375 -
(c) Test material, JPR Blue 100 (3 mg/l) with inoculum	} O2depletion (mg O2/l) } % degradation	0.025 1	0.000 0	0.000 0
(d) Standard material, Sodium benzoate (3 mg/l) with inoculum	} O2depletion (mg O2/l) } % degradation	3.475 69	3.600 72	4.225 84

 

i) Test and Standard materials

Oxygen depletion = (a – c or d) – (a – b)

 

ii) Inoculated and non-inoculated culture medium

Oxygen depletion = initial O₂concentration – measured value

 

iii) % Degradation =                Oxygen depletion

                                   ---------------------------------------------- x 100

                                      Conc. of material x ThOD NO₃

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

RESULTS
TEST CONCENTRATION: 3 mg/l
OXYGEN DEPLETION (28 Days): 0.00 mg O2/l
THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O2/l
PERCENTAGE BIODEGRADATION (28 Days): 0%

Executive summary:

TEST TYPE: Ready biodegradation; Closed bottle Test

TEST MATERIAL: JPR Blue 100

METHOD: Closed Bottle Test according to OECD Guideline No 301D referenced as Method C.6 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC)

DURATION: 28 days.

TEMPERATURE: 21 °C.

 

RESULTS

TEST CONCENTRATION: 3 mg/l

OXYGEN DEPLETION (28 Days): 0.00 mg O₂/l

THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O₂/l

PERCENTAGE BIODEGRADATION (28 Days): 0%

Description of key information

OXYGEN DEPLETION (28 Days): 0.00 mg O₂/l

THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O₂/l

PERCENTAGE BIODEGRADATION (28 Days): 0%

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

TEST TYPE: Ready biodegradation; Closed bottle Test

TEST MATERIAL: JPR Blue 100

METHOD: Closed Bottle Test according to OECD Guideline No 301D referenced as Method C.6 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC)

DURATION: 28 days.

TEMPERATURE: 21 °C.

 

RESULTS

TEST CONCENTRATION: 3 mg/l

OXYGEN DEPLETION (28 Days): 0.00 mg O₂/l

THEORETICAL OXYGEN DEMAND (ThOD): 4.68 mg O₂/l

PERCENTAGE BIODEGRADATION (28 Days): 0%

[Type of water: freshwater]