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EC number: 202-443-7 | CAS number: 95-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.05.2018 - 24.05.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1981
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- accredited by DAkkS
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from an urban municipal waste water treatment facility was used as inoculum. It was washed with tap water in the laboratory. For this purpose, the activated sludge was transferred into a large beaker and was allowed to settle for a few minutes. The aquous supernatant was removed by decanting. Subsequently, slurrying of the residue was conducted with tap water. This process was repeated for 3 times and served as purification of the activated sludge from possible inhibitors. After the last decantation, no slurrying was performed so that a higher concentration was achieved. Subseqently, the dry matter was determined and adjusted to 3,2% by a renewed addition of tap water (the value was determined by a renewed measurement of the dry matter). The prepared activated sludge like this was used as inoculum and supplied with oxygen during the whole test duration by permanent stirring.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2°C
- pH:
- see table 2
- Dissolved oxygen:
- see table 2
- Nominal and measured concentrations:
- 6,4 mg/l, 16 mg/l, 40 mg/l, 100 mg/l, 250 mg/l
- Details on test conditions:
- The test was performed in single samples with a test volume of 500 ml. Solely, the control sample was performed in dupilcate. All samples contained 16 ml synthetic wastewater (for the composition see table 1) and 200 ml inoculum with a dry matter of 3.2%. In the control samples 284 ml tap water was added and in the test samples 284 ml methlydroquinone in different concentrations. The reference sample contained tap water and mercury chloride in a concentrion of 10 mg/l.
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 22.15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 6.79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 72.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- see table 2 and 3
- Validity criteria fulfilled:
- yes
- Remarks:
- The validity of the test is given since the oxygen consumption in the control samples only deviate by 4.3%. Moreover, the respiration inhibition in the reference sample reached a value of 93%.
- Conclusions:
- The respiration inhibition of activated sludge for methylhydroquinone was investigated using a respirometer in a study according to OECD test guideline 209. The 3 h EC50 was determined to be 22.15 mg/L (nominal).
- Executive summary:
The OECD 209 activated sludge, respiration inhibition test was performed as a method for assessing the potential impact of the test substance on wastewater treatment system. Optimal contact between the test substance and test medium was ensured applying continuous aeration during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for 10 minutes. Five concentrations, ranging from 6.4 to 250 mg/l were tested.
A statistically significant inhibition of the respiration rate of the activated sludge was recorded and an EC50 of 22.15 mg/l was determined by linear regression.
Reference
Table 2: Oxygen content and pH-value of control samples, test samples and reference samples
t (min) |
O2 content in mg/l |
|||||||
control samples |
test samples |
reference sample |
||||||
C1 |
C2 |
6,4 mg/l |
16 mg/l |
40 mg/l |
100 mg/l |
250 mg/l |
||
0,0 |
7,96 |
7,80 |
6,40 |
8,14 |
8,09 |
8,51 |
8,27 |
8,48 |
0,5 |
7,25 |
7,51 |
6,03 |
7,96 |
7,97 |
8,48 |
8,21 |
8,46 |
1,0 |
6,81 |
7,22 |
5,74 |
7,80 |
7,84 |
8,46 |
8,14 |
8,45 |
1,5 |
6,47 |
6,91 |
5,49 |
7,66 |
7,75 |
8,31 |
8,06 |
8,43 |
2,0 |
6,19 |
6,65 |
5,24 |
7,54 |
7,65 |
8,21 |
8,00 |
8,41 |
2,5 |
5,91 |
6,37 |
4,97 |
7,41 |
7,57 |
8,17 |
7,94 |
8,37 |
3,0 |
5,65 |
6,06 |
4,72 |
7,27 |
7,37 |
8,12 |
7,87 |
8,36 |
3,5 |
5,39 |
5,78 |
4,45 |
7,14 |
7,38 |
8,06 |
7,80 |
8,34 |
4,0 |
5,13 |
5,49 |
4,19 |
7,01 |
7,30 |
8,02 |
7,74 |
8,31 |
4,5 |
4,86 |
5,20 |
3,92 |
6,86 |
7,19 |
7,97 |
7,66 |
8,30 |
5,0 |
4,59 |
4,93 |
3,66 |
6,73 |
7,11 |
7,91 |
7,62 |
8,27 |
5,5 |
4,32 |
4,64 |
3,39 |
6,61 |
7,03 |
7,87 |
7,54 |
8,25 |
6,0 |
4,06 |
4,35 |
3,13 |
6,47 |
6,94 |
7,82 |
7,48 |
8,24 |
6,5 |
3,78 |
4,06 |
2,86 |
6,34 |
6,84 |
7,77 |
7,41 |
8,20 |
7,0 |
3,53 |
3,75 |
2,60 |
6,20 |
6,76 |
7,72 |
7,35 |
8,19 |
7,5 |
3,26 |
3,47 |
2,33 |
6,06 |
6,68 |
7,67 |
7,27 |
8,17 |
8,0 |
2,98 |
3,19 |
2,07 |
5,91 |
6,60 |
7,62 |
7,22 |
8,13 |
8,5 |
2,71 |
2,90 |
1,81 |
5,78 |
6,52 |
7,57 |
7,15 |
8,11 |
9,0 |
2,45 |
2,58 |
1,57 |
5,64 |
6,43 |
7,52 |
7,09 |
8,09 |
9,5 |
2,18 |
2,32 |
1,32 |
5,50 |
6,34 |
7,48 |
7,03 |
8,07 |
10,0 |
1,92 |
2,02 |
1,06 |
5,36 |
6,27 |
7,43 |
6,97 |
8,05 |
|
||||||||
pH |
7,28 |
7,48 |
7,49 |
7,72 |
7,74 |
7,74 |
7,52 |
7,74 |
Table 3: Oxygen consumption of control samples, test samples and reference samples in mg O2/l*h and %
Sample |
concentration (mg/l) |
Oxygen consumption |
||
mg O2/l*h |
mean |
% |
||
control |
0 |
36,24 |
35,46 |
100 |
0 |
34,68 |
|||
test sample |
6,4 |
32,04 |
|
90,36 |
16 |
16,68 |
47,04 |
||
40 |
10,92 |
30,80 |
||
100 |
6,48 |
18,27 |
||
250 |
7,8 |
22,00 |
||
mercury chloride |
10 |
2,58 |
7,28 |
Description of key information
The respiration inhibition of activated sludge for methylhydroquinone was investigated using a respirometer in a study according to OECD test guideline 209. The EC50 (3 h) was determined to be 22.15 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 22.15 mg/L
Additional information
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