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EC number: 212-611-1 | CAS number: 831-82-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- An abiotic Control for the reference item was performed during this study although it was not planned in the study plan.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch 20180329 (Source Lead)
- Expiration date of the lot/batch: March 2019
- Purity test date: March 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: romm temperature 20 +/-10°C
- Stability under test conditions: stable
- Solubility in water : 0.19 to 0.26 g/L - Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: directly diluted
- Controls: Yes
- Chemical name of vehicle : osmosed water
- Concentration of vehicle in test medium : 10 - 40 - 160 - 640 and 2560 mg/L
- Evidence of undissolved material: the test item is poorly soluble in water - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
FCBA
- Name and location of sewage treatment plant where inoculum was collected: STEP de Lille, 33290 BLANQUEFORT - FRANCE
- Method of cultivation: A filtration through a fine sieve (around 1 mm) was performed in order to remove coarse particles. After removal of any coarse particles, the sludge was washed by decantation in osmosed water and test medium ( 50/50 (v/v) to adjust concentration of dry extract around 3 g/L. The activated sludge was continuously aerated until usage in the test and fed daily with 50 mL/L synthetic sewage feed for one or two additional days.
- Test medium : mineral mediium (Na2HPO4, 2H2O, K2HPO4, NH4Cl, KH2PO4, CCl2, 2H2O, MGSO4, 7H2O, FeCl3, 6H20)
- Initial biomass concentration: 1.45 g suspended solids/L in this test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 d
- Hardness:
- No data
- Test temperature:
- 20 +/-2°C
- pH:
- End of the test : 8.2 to 8.6
- Salinity:
- Not relevant
- Conductivity:
- Not relevant
- Nominal and measured concentrations:
- Nominal : 10 - 40 - 160 - 640 and 2560 mg/L
- Details on test conditions:
- The test was carried out into 1000 mL glass flasks containing 500 mL as below:
- 16.0 ml synthetic sewage feed (Peptone, Meat Extract, Ura, NCl, CaCl2-2H2O, MgSO4-7H20, K2HPO4, ultrapure water)
- Adequate weighing of test item
- osmosed water (final volume: 500 mL)
- 250 mL of sludge suspension
Replicates: Six replicates for controls (Control a to Control f)
Two replicates for abiotic controls
Three replicates (a, b and c) per treatment (1 to 5) for the test item
One replicate per treatment for the reference item (3,5 DCP)
The concentrations for the 3,5 DCP were : 0.5 – 1.6 – 5.1 – 16.40 and 52.4 mg/L
At the start of the test, the first control (Control a) was prepared and covered with parafilm. Then every 10 -15 minutes the others flasks were prepared in turn in the same conditions. Finally, the last control (Control f) was prepared.
All suspensions were aerated with purified air (0.5 to 1 L/min), and stirred during 3 hours at 20 ± 2°C. Then, each suspension was poured into Karlsruher flask (following the order preparation). The Oxygen was measured every minute, during 10 minutes, with a Dissolved Oxygen Sensor (WTW StirrOx G) integrated Stirrer, inserted into the Karlsruher flask
EFFECT PARAMETERS MEASURED : Activated sludge respiration inhibition, measured as oxygen consumption rate (dissolved oxygen concentration) - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 43.2 - < 296.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 165.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 40 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- -
- Results with reference substance (positive control):
- - Results with reference substance valid : Yes
- Relevant effects levels : EC50 : 13.7 mg/L ; oxygen uptake in the control : 38.8 mg O2/ g sludge/h ; Coefficient of varation of O2 : 0.0 to 14.6% - Validity criteria fulfilled:
- yes
- Conclusions:
- This study was performed using five concentrations, ranging from 10 to 2560 mg/L nominal concentrations. The dry matter of the activated sludge was determined as 2.9 g suspended solids/L, giving a concentration of 1.45 g suspended solids/L in this test. The validity criteria for OECD 209 were successful. And the nominal concentrations of 4-PhenoxyPhenol are :
- CE50-3h = 165.4 mg/L [43.2 - 296.6]
- NOEC = 10 mg/L
- LOEC = 40 mg/L - Executive summary:
An OECD 209 was performed on 4 -PhenoxyPhenol using five concentrations, ranging from 10 to 2560 mg/L nominal concentrations. The dry matter of the activated sludge was determined as 2.9 g suspended solids/L, giving a concentration of 1.45 g suspended solids/L in this test. The validity criteria for OECD 209 were successful. And the nominal concentrations of 4-PhenoxyPhenol are :
- CE50-3h = 165.4 mg/L [43.2 - 296.6]
- NOEC = 10 mg/L
- LOEC = 40 mg/L
Reference
O2 consumption and inhibition
Treatment |
Name |
O2consumption in mg O2/(L*h) |
% inhibition |
pH (end of the test) |
Inoculum Control |
Control a |
51.00 |
/ |
8.4 |
Control b |
52.50 |
/ |
8.4 |
|
Control c |
61.50 |
/ |
8.3 |
|
Control d |
60.00 |
/ |
8.2 |
|
Control e |
57.60 |
/ |
8.2 |
|
Control f |
55.20 |
/ |
8.3 |
|
Abiotic Control reference item |
Abiotic Control 3,5 DCP |
/ |
/ |
7.0 |
Reference Item |
3,5 DCP Conc 1 |
55.5 |
1.42 |
8.3 |
3,5 DCP Conc 2 |
54.0 |
4.09 |
8.4 |
|
3,5 DCP Conc 3 |
48.0 |
14.74 |
8.4 |
|
3,5 DCP Conc 4 |
22.7 |
59.68 |
8.4 |
|
3,5 DCP Conc 5 |
7.5 |
85.79 |
8.4 |
|
Abiotic Control Test item |
Abiotic Control Test item a |
/ |
/ |
7.0 |
Test Item |
1a |
62.00 |
- 10.1 |
8.2 |
1b |
62.00 |
- 10.1 |
8.2 |
|
1c |
54.00 |
4.1 |
8.3 |
|
2a |
42.00 |
25.4 |
8.3 |
|
2b |
43.00 |
23.6 |
8.3 |
|
2c |
36.00 |
36.1 |
8.4 |
|
3a |
8.00 |
85.8 |
8.4 |
|
3b |
3.43 |
93.9 |
8.5 |
|
3c |
3.33 |
94.1 |
8.4 |
|
4a |
0.00 |
100.0 |
8.6 |
|
4b |
0.67 |
98.8 |
8.6 |
|
4c |
0.00 |
100.0 |
8.6 |
|
5a |
0.67 |
98.8 |
8.6 |
|
5b |
0.00 |
100.0 |
8.6 |
|
5c |
0.67 |
98.8 |
8.6 |
|
Abiotic Test item |
Abiotic Test item b |
/ |
/ |
7.0 |
Description of key information
- CE50-3h = 165.4 mg/L [43.2 - 296.6]
- NOEC = 10 mg/L
- LOEC = 40 mg/L
An OECD 209 was performed on 4 -PhenoxyPhenol using five concentrations, ranging from 10 to 2560 mg/L nominal concentrations. The dry matter of the activated sludge was determined as 2.9 g suspended solids/L, giving a concentration of 1.45 g suspended solids/L in this test. The validity criteria for OECD 209 were successful. And the nominal concentrations of 4-PhenoxyPhenol are :
Key value for chemical safety assessment
- EC50 for microorganisms:
- 165.4 mg/L
- EC10 or NOEC for microorganisms:
- 10 mg/L
Additional information
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