Registration Dossier
Registration Dossier
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EC number: 812-508-7 | CAS number: 1093112-19-6
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 05. May 2003 to 08. Aug. 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 203
- Version / remarks:
- Daphnia magna for 48 h in 5 different concentrations of test substance, daily renewal of test medium, pH adjusted at 7.5 at each renewal
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- certified by Ministerium für Umwelt, Raumordnung und Landwirtschaft des s landes Nordrhen-West´falen, des >L
Test material
- Reference substance name:
- Aluminium sulphate
- EC Number:
- 233-135-0
- EC Name:
- Aluminium sulphate
- Cas Number:
- 10043-01-3
- Molecular formula:
- Al.3/2H2O4S
- IUPAC Name:
- aluminium sulphate
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Aluminium sulphate-14-hydrate
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Water phase samples were taken from the freshly prepared test solutions
One subsample of 20mL acidified with 0.2mL 14M nitric acid (pH<2): unfiltered sample containing total aluminium
A second subsample of 20mL passed through a rinsed 0.45 µm-membrane filter and then acidified: dissolved metal fraction
Test solutions
- Vehicle:
- yes
- Remarks:
- reconstituted water according to annex 2 of guideline 203
- Details on test solutions:
- reconstituted water, no feeding and no aeration during the test,
pH-values (adjusted at 7.5) and oxygen concentrations controlled at test star, after 24h and test end
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna, clone V, 4-24h old, supplied from Umweltbundesamt and bred in the laboratory
separated by sieving, held in batches of 30 - 50 animals at room temperature in ca. 1800 mL purified drinking water,
daily fed with alga suspension and LiquizellR accroding to EEC-Guideline
water changed once a week
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- semi-static, water chnged after 24h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- 11.76g CaCl2*2H2O/L
sum of calcium and magnesium ions is 2.5mmol/L, proportion Ca.Mg ions is 4:1 - Test temperature:
- 20.0 - 20.1°C
- pH:
- adjusted to 7.5, 7.7-8.0 measured
- Dissolved oxygen:
- 93% - 100%
- Salinity:
- freshwater, 2.59g NaHCO3/L
- Conductivity:
- not measured
- Nominal and measured concentrations:
- nominal: 10.0/20.0/40.0/80.0/160.0 mg test substance
nominal: 0.900/1.80/3.60/7.20/14.4 mg Al/L
measured: 0.003/ 0.172/0.176/0.166/0.107/0.115 mg dissolved Al/L (mean) - Details on test conditions:
- The range of dissolved Al-concentrations was between 0.08-0.27mg/L, irrespective of the nominal total Al-concentrations.
This seems to correspond to the Al-dissolution capacity of the test medium (buffered to prevent dramatic changes in pH)
Since in the controls a measurable unstructured spectroscopic background was detected by ICP-OES, all data were corrcted
No background was detected by the membrane-filtered samples analysed by ICP-MS - Reference substance (positive control):
- yes
- Remarks:
- sensitivity checks using K2Cr2O7
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Sensitivity checks between 04. Apr. 2003 and 13. May 2003 using K2Cr2O7: EC50: 1.0 - 1.3mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- no immobility of Daphnids could observed after 48h: NOEC >/= 160 mg aluminium sulphate-14-hydrate /L
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