2,2',4,4',6,6'-hexanitrostilbene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2020

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,2',4,4',6,6'-hexanitrostilbene

EC Number:

243-494-5

EC Name:

2,2',4,4',6,6'-hexanitrostilbene

Cas Number:

20062-22-0

Molecular formula:

C14H6N6O12

IUPAC Name:

1,3,5-trinitro-2-[(E)-2-(2,4,6-trinitrophenyl)ethenyl]benzene

Test material form:

solid: crystalline

Specific details on test material used for the study:

Batch no. 18909-1
Composition 100%
Expiry date 27/2/2028

Method

Target gene:

histidine

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102

Metabolic activation:

with and without

Metabolic activation system:

S9-mix

Test concentrations with justification for top dose:

Based on the results of the first experiment, the test item was tested up to concentrations of
5000 µg/plate in the presence and absence of S9-mix in all bacterial strains using the pre-
incubation method.

Vehicle / solvent:

DMSO

Results and discussion

Test results

Species / strain:

S. typhimurium, other: All tested strains

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Additional information on results:

The test item showed slight precipitates at the highest test item concentration
(5000 µg/plate) in all bacterial strains. No precipitates could be observed on the plates at
any of the lower tested concentrations .No cytotoxic effect towards the bacterial strains could
be observed in the presence and the absence of metabolic activation.
The results of this experiment showed that none of the tested concentrations showed a
significant increase in the number of revertants in all tested strains, in the presence and the
absence of metabolic activation.

Applicant's summary and conclusion

Conclusions:

Based on the results of this study it is concluded that HEXANITROSTILBENE is not
mutagenic in the Salmonella typhimurium strains TA98, TA100, TA102, TA1535 and
TA1537 in the presence and absence of metabolic activation under the experimental
conditions in this study.