3-acetamidoanilinium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14-18 January 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-acetamidoanilinium chloride

EC Number:

210-682-3

EC Name:

3-acetamidoanilinium chloride

Cas Number:

621-35-2

Molecular formula:

C8H10N2O.ClH

IUPAC Name:

3-acetamidoanilinium chloride

Test material form:

solid: bulk

Test solutions

Details on test solutions:

Test item was direct dispersed in water.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.79 ± 0.3 cm
- Weight at study initiation: 0.86 ± 0.13 g

ACCLIMATION
- Health during acclimation: 0 % mortality in the 7 days prior to the start of the test.
- Medication: none

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes
- N. organisms per replicate: 10.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 1 replicate.
- Biomass loading rate: 0.43 g bodyweight/litre

TEST MEDIUM / WATER PARAMETERS
- Dilution water: dechlorinated tap water (Sodium thiosulphate).
- Temperature: 14 °C
- Hardness: approximately 50 mg/l as CaCO3

RANGE FINDING
- Concentration: 1.0, 10 and 100 mg/l (nominal)
- N. organisms per replicate: 2

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Main test

Concentration (mg/l )	Cumulative mortality (initial population: 10)
	24 hrs	48 hrs	72 hrs	96 hrs
Control	0	0	0	0
Test item 100	0	0	0	0
Test item 100	0	0	0	0

Applicant's summary and conclusion

Conclusions:

LC50 (96h) > 100 mg/l (nominal)

Executive summary:

The short-term toxicity to fish of the test item has been investigated using Rainbow trout (Oncorhynchus mykjss). A range-finding experiment was conducted at the nominal concentrations of 1.0, 10 and 100 mg/l. The main test was conducted at the nominal concentration of 100 mg/l; two replicates were assayed. Blank control was also been tested.

No deaths weres recorded in the main experiment replicates.

Conclusion

LC50 (96h) > 100 mg/l (nominal)