Benzenesulfonic acid, mono-C10-13-alkyl derivs., compds. with diethanolamine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. II. Results with 20 chemicals

Author:

Loveday KS, et al.

Year:

1989

Bibliographic source:

Environ. Mol. Mutagen., 13, 60-94

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 479 (Genetic Toxicology: In Vitro Sister Chromatid Exchange Assay in Mammalian Cells)

GLP compliance:

not specified

Type of assay:

sister chromatid exchange assay in mammalian cells

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-iminodiethanol

EC Number:

203-868-0

EC Name:

2,2'-iminodiethanol

Cas Number:

111-42-2

Molecular formula:

C4H11NO2

IUPAC Name:

2,2'-iminodiethanol

Method

Species / strain

Species / strain / cell type:

Chinese hamster Ovary (CHO)

Metabolic activation:

with and without

Metabolic activation system:

rat liver S9 (Aroclor 1254-induced)

Test concentrations with justification for top dose:

150, 500, 1500 µg/mL

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: cyclophosphamide (with S9); mitomycin C (without S9)

Statistics:

Statistical analyses were conducted on the slopes of the dose-response curves and the individual dose points.

Results and discussion

Test results

Key result

Species / strain:

Chinese hamster Ovary (CHO)

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

1500 µg/ml

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

Results summary:

The test substance did not induce sister chromatid exchanges in CHO cell and thus, showed no mutagenic potential under the chosen test condition.

The sensitivity of the test system was verified since the positive control substances showed the expected positive reactions

Detailed results:

Induction of sister chromatid exchanges in Chinese hamster ovary cells
without metabolic activation:
       

Treatment (µg/mL)	Cells	No. SCE	SCE/chromosome	SCE/cells
Medium	50	465	0.44	9.3
Mitomycin C (0.002)	50	727	0.69	14.5
Mitomycin C (0.010)	10	319	1.54	31.9
DEA (150)	50	459	0.43	9.2
DEA (500)	50	487	0.46	9.7
DEA (1500)	50	469	0.45	9.4

                                         
with metabolic activation:
                                                        

Treatment (µg/mL)	Cells	No. SCE	SCE/chromosome	SCE/cells
Medium	50	518	0.51	10.4
Cyclophosphamide (0.5)	50	1053	1.03	21.1
Cyclophosphamide (2.5)	10	592	2.94	59.2
DEA (150)	50	505	0.49	10.1
DEA (500)	50	539	0.53	10.8
DEA (1500)	50	565	0.56	11.3