Registration Dossier
Registration Dossier
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EC number: 254-599-0 | CAS number: 39711-79-0
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to fish:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.
Short term toxicity to aquatic invertebrate:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium butanedioate. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’ media with continuous 72 hrs stirring and the concentration of stock solution was analytically detected and then the test solution was prepared for achieving the test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively.
The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 50 mg/L but < 100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteriato aquaticuatic classification of the substance, it is concluded that exhibits short term toxicity to Daphnia.
After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be> 50 mg/L but < 100 mg/L. Based on the EC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.
Additional information
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