Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-219-6 | CAS number: 79-69-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-methyl-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one
- EC Number:
- 204-846-3
- EC Name:
- 3-methyl-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one
- Cas Number:
- 127-51-5
- Molecular formula:
- C14H22O
- IUPAC Name:
- 3-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)but-3-en-2-one
Constituent 1
- Specific details on test material used for the study:
- Name: Methyl Ionone Alpha Iso 60
Chemical name: 4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-Methyl-3-Buten-2-one
Description: Pale yellow liquid
Storage: Room temperature in the dark
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- The stock solution used in this study was prepared dispersing 0.18 g test substance (weighed out using the Mettler AE200 balance in 1.0 litre Elendt M7 medium to produce a 100 mg/l as α-iso isomer, 180 mg/l as commercial sample stock. The dispersion was achieved by mixing this stock using a high shear mixer for approximately 5 minutes at 12-14000 rpm. The test solutions were prepared by adding known volumes of stock to 2000 ml Elendt medium.
These test solutions were prepared 24 hours before the Daphnia were added to allow the test substance to equilibrate.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna were cultured in the laboratory according to the procedures described in SOP 019. Cultures were maintained in a synthetic medium with the same chemical composition and similar pH and dissolved oxygen concentration as that used to prepare test solutions. Culture medium consists of 800 ml Elendt M7 medium. The cultures are fed daily with cells of the green algae Chlorella vulgaris. Cultures are reset using 10 neonates, when they become gravid their numbers are reduced from 10 to 5 and the production of neonates results in their daily removal.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- The total hardness (as mg/l CaCO3) of selected solutions were measured at 0 and 48 hours using the appropriate methods. Total hardness (as mg/l CaCO3): 247 - 251
- Test temperature:
- The temperature of selected solutions were measured at 0 and 48 hours using the appropriate methods. Temperature: 19.0 - 21.5 °C
- pH:
- The pH of selected solutions were measured at 0 and 48 hours using the appropriate methods. pH: 7.4 - 7.6
- Dissolved oxygen:
- The dissolved oxygen concentration of selected solutions were measured at 0 and 48 hours using the appropriate methods. Dissolved oxygen concentration: 6.9 - 8.4 mg/l
- Nominal and measured concentrations:
- Nominal concentrations: 0.0, 1.8, 5.8, 10, 18 and 32 mg/l
Concentration of test substance based on mean measured TOC: 0.0, 1.8, 4.0, 7.3, 12 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of Methyl Ionone Alpha Iso 60 to the freshwater cladoceran Daphnia magna was determined according to OECD Guideline 202 and EEC Directive 92/69/EEC L383 A.
Daphnia were exposed to a nominal range of concentrations 1.8 to 32 mg/l as the commercial sample. Analysis of the test substance, during the period of the test, remained relatively stable, however, the calculated concentrations based on TOC measurements were in the range of 0 - 41% of nominal.
The EC50s of the commercial sample based on mean measured TOC concentrations and calculated by the Binomial and Probit methods respectively, are given below:
48 hours: EC50 = 3.7 mg/l, 95% confidence limits 3.0 - 4.5
NOEC = 1.8 mg/l
Lowest conc. causing 100% mortality 7.3 mg/l
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.