Isobutyl (S)-2-chloropropionate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study according to the German National Standard (DIN).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl (S)-2-chloropropionate

EC Number:

280-349-5

EC Name:

Isobutyl (S)-2-chloropropionate

Cas Number:

83261-15-8

Molecular formula:

C7H13ClO2

IUPAC Name:

isobutyl (S)-2-chloropropanoate

Constituent 2

Reference substance name:

Isobutyl-2-chloropropionate

IUPAC Name:

Isobutyl-2-chloropropionate

Details on test material:

- Name of test material (as cited in study report): L-Chlorpropionsaeureisobutylester
- no further details on test substance characteristics are given

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Concentration of the stock solution: 200 mg/l nominal and 100 mg/L Tween80 (Merck).
- Eluate: The stocksolution was stirred for 60 min at 20 ± 2 °C

- Differential loading:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80 (dispersant)
- Concentration of vehicle in test medium (stock solution and final test solution): stock solution: 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUSS
- Source: Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been breeded from 1978 in the Laboratories of BASF.
- Age at study initiation: 2 - 24 hours old

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.7 ± 0.5 mmol/L

Test temperature:

19 - 21°C

pH:

8.0 ± 0.5

Dissolved oxygen:

7.5 - 9.1 mg/L

Nominal and measured concentrations:

Nominal: 6.25, 12.5, 25, 50, 100 and 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 20 ml glass tubes with flat bottom, 10 ml test volume
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/L
Ratio Ca/Mg ions: about 4:1
pH value: 7.5 - 8.5
Conductivity: 600 - 700 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light (about 2-7 microeinstein/m*m*s (400-750 nm))


EFFECT PARAMETERS MEASURED
Observations: mobile daphnids after 24 and 48 h
Measurements: Temperature, pH and oxygen content after 0 and 48 h


TEST CONCENTRATIONS
Nominal test concentrations: 6.25, 12.5, 25, 50, 100 and 200 mg/L. A control was tested in parallel.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

73 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Effect concentrations after 24 h:
EC0 = 50 mg/L
EC50 = 87 mg/L
EC100 = 200 mg/L