3-methylbut-2-en-1-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test method comparable to guideline study, and in accordance with accepted German national standards.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methylbut-2-en-1-ol

EC Number:

209-141-4

EC Name:

3-methylbut-2-en-1-ol

Cas Number:

556-82-1

Molecular formula:

C5H10O

IUPAC Name:

3-methylbut-2-en-1-ol

Details on test material:

- Name of test material (as cited in study report): 3-Methylbuten-2-ol-1 (Prenol)
- Lot/batch No.: B 2391
- Analytical purity: 99 %
Water solubility: 90 g/l

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The test substance was directly added to test water.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name:  Golden orfe 
- Length at study initiation (length definition, mean, range and SD):  5.7 - 7.2 cm    
- Weight at study initiation (mean and range, SD): 1.5 - 4.4 g
- Feeding during test: 1 day before exposure

ACCLIMATION
- Acclimation period: 3 day
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

approx. 2.5 mmol/l

Test temperature:

21 °C

pH:

~ 8

Nominal and measured concentrations:

Nominal test concentrations: 10, 21.5, 46.4, 100, 215, 464 mg/l and a control 

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.9 g Fish/ l test water

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h (light:dark)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Determination of mortality and symptoms were recorded after 1, 4, 24, 48,  72, 96 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

21.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Volatility screening test:
Based on TOC measurements the recovery rate at the end of the exposure  period was 93 % for the fish test. This shows that the test substance concentration remained nearly constant  under the conditions of the eco-toxicity test and therefore the effect  values based on nominal concentrations can be used for the hazard  assessment without limitations.

Reported statistics and error estimates:

The LC50 was statistically evaluated as the geometrical mean of the LC0  value (21.5 mg/l) and the LC100 value (100 mg/l).